Group of Genetics, Breeding and Biochemistry of Brassica Crops, Mision Biologica de Galicia (MBG), CSIC, Pontevedra, Spain.
Pest Manag Sci. 2023 Feb;79(2):803-810. doi: 10.1002/ps.7242. Epub 2022 Nov 1.
Plants defend themselves from insect feeding by activating specific metabolic pathways. We performed a metabolomic analysis to compare the metabolome reorganization that occurs in the leaves of two genotypes of cabbage (one partially resistant and one susceptible) when attacked by Mamestra brassicae caterpillars.
The comparison of the metabolomic reorganization of both genotypes allowed us to identify 43 metabolites that are specifically associated with the insect feeding response in the resistant genotype. Of these, 19% are lipids or lipid-related compounds, most of which are modified fatty acids. These include glycosylated, glycerol-binding and oxidized fatty acids, the majority being associated with the oxylipin pathway. Some of the identified lipids are unlikely to be produced by plants and may be the result of biochemical reactions in the caterpillar oral secretions. A further 16% are phenylpropanoids. Interestingly, some phenylpropanoids were not present in the susceptible genotype, making them possible candidates for specific resistance-related compounds.
Our results suggest that glucosinolates do not have a clear role in the resistance to M. brassicae feeding on cabbage. Using an untargeted metabolomics approach, we associated the regulation of metabolic pathways related to lipid signalling and phenylpropanoid compounds with the resistance to this pest. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
植物通过激活特定的代谢途径来保护自己免受昆虫取食。我们进行了代谢组学分析，比较了当小菜蛾毛虫攻击两种甘蓝基因型（一种部分抗性，一种易感性）时，叶片中发生的代谢组重排。
比较两种基因型的代谢组重排，使我们能够鉴定出 43 种与抗性基因型中昆虫取食反应特异性相关的代谢物。其中，19%是脂质或与脂质相关的化合物，其中大部分是修饰脂肪酸。这些包括糖基化、甘油结合和氧化脂肪酸，大多数与类脂氧素途径有关。鉴定出的一些脂质不太可能由植物产生，可能是毛虫口腔分泌物中生化反应的结果。另有 16%是苯丙烷类化合物。有趣的是，一些苯丙烷类化合物在易感性基因型中不存在，它们可能是特定抗性相关化合物的候选物。
我们的研究结果表明，硫代葡萄糖苷在甘蓝对小菜蛾取食的抗性中没有明显作用。本研究采用非靶向代谢组学方法，将与脂质信号和苯丙烷化合物代谢途径相关的代谢调控与对该害虫的抗性联系起来。© 2022 作者。虫害管理科学由 John Wiley & Sons Ltd 代表化学工业协会出版。
