Department of Pharmaceutical Technology, Jordan University of Science and Technology, Irbid, Jordan.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Ann Med. 2022 Dec;54(1):2796-2804. doi: 10.1080/07853890.2022.2131282.
Notable emergence of multidrug-resistant bacteria has become increasingly problematic worldwide. Most patients with cystic fibrosis (CF) suffer from chronic persistent infections with frequent occurrence of acute exacerbations. Routine screening of bacterial strains, epidemiological characteristics, and resistance patterns are particularly useful for patient management and maintenance of infection control procedures.
In this study, 43 pharyngeal samples were taken from patients with CF. Microbiological bacterial culture and identification, antimicrobial susceptibility testings, biofilm formation, including minimum biofilm eradication concentration (MBEC) and PCR for detecting resistance genes were performed.
All samples were positive for bacterial growth. The predominant species were (41.86%; = 18) and (39.53%; = 17). 30% of isolated bacteria were multidrug-resistant, resisting high concentrations of tested antibiotics. Among the 42 biofilm-forming isolates, 23.8% ( = 10) were strong biofilm formers. The occurance of resistance genes varied with detected in 71% ( = 17) of all Gram-negative isolates and found in 61% ( = 11) of all strains.
The majority of isolated bacteria were and . The high frequency of antimicrobial resistance, the presence of resistance genes, and biofilm formation highlight the challenge in treatment and infection control measures in patients with CF.KEY MESSAGES and are the most prevalent pathogens found in patients with CF in Jordan.Detection of antimicrobial resistance genes in patients with CF confirms that antimicrobial resistance patterns must always be monitored.Biofilm formation significantly increases the tolerance of bacteria to antimicrobial agents.
多药耐药菌的显著出现已成为全球日益严重的问题。大多数囊性纤维化（CF）患者患有慢性持续性感染，经常发生急性加重。常规筛选细菌株、流行病学特征和耐药模式对患者管理和感染控制程序的维护特别有用。
本研究从 CF 患者中采集了 43 份咽拭子样本。进行了微生物细菌培养和鉴定、抗菌药物敏感性试验、生物膜形成，包括最低生物膜清除浓度（MBEC）和检测耐药基因的 PCR。
所有样本均呈细菌生长阳性。主要物种为 （41.86%； = 18）和 （39.53%； = 17）。30%的分离细菌为多药耐药菌，对高浓度测试抗生素有耐药性。在 42 个生物膜形成分离株中，23.8%（ = 10）为强生物膜形成者。耐药基因的发生率因 而有所不同，所有革兰氏阴性分离株中有 71%（ = 17）检测到 ，所有 菌株中有 61%（ = 11）检测到 。
大多数分离的细菌为 和 。抗菌药物耐药率高、耐药基因存在和生物膜形成突出了 CF 患者治疗和感染控制措施的挑战。
在约旦 CF 患者中，最常见的病原体为 和 。在 CF 患者中检测到抗菌药物耐药基因证实，必须始终监测抗菌药物耐药模式。生物膜形成显著增加了细菌对抗菌药物的耐受性。
