School of Pharmacy, Qingdao University, Qingdao266071, P.R. China.
Key Laboratory of Functional Polymer Materials of Ministry of Education, State Key Laboratory of Medicinal Chemical Biology and Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin300071, P.R. China.
ACS Appl Mater Interfaces. 2022 Nov 2;14(43):48426-48437. doi: 10.1021/acsami.2c13420. Epub 2022 Oct 20.
Wound healing involves multiple stages of body responses, including hemostasis, inflammation, cell proliferation, and tissue remodeling. New material design satisfying all demands throughout different stages of wound healing is cherished but rarely discussed. Here we introduce all-aqueous multiphase microfluidics as a novel strategy to fabricate self-assembled, multifunctional alkylated chitosan/alginate microcapsules (SAAMs) as novel therapeutic materials for rapid blood coagulation and wound healing. SAAMs are structurally distinguished by their ultrathin shells with polycationic surface for rapid activation of clotting cascade and their internal porous dextran-rich cores for fast absorption of blood and exudate. These features endow SAAMs with excellent hemostatic properties for acute hemorrhage. Moreover, the alkylated chitosan within the microcapsules exhibits persistent antimicrobial activities against bactericidal infections due to their amphiphilic and cationic surfaces. Besides, cytokines can be safely loaded into the organic-solvent-free microcapsules and released precisely to promote the proliferation of epidermal cells, supporting the subsequent development of granulation tissue and suppression of inflammation in the last stages of wound healing. With the ability to fabricate size-tailored soft microcapsules and to realize time-sequential functions for tissue repairing, the presented "all-aqueous microfluidics generation of multifunctional bioactive SAAMs" create a versatile and robust paradigm for wound treatment.
伤口愈合涉及多个身体反应阶段，包括止血、炎症、细胞增殖和组织重塑。满足不同伤口愈合阶段所有需求的新材料设计是人们所渴望的，但很少有讨论。在这里，我们介绍了全水多相微流控技术，作为一种制备自组装多功能烷基化壳聚糖/海藻酸盐微胶囊（SAAMs）的新策略，用于快速凝血和伤口愈合的新型治疗材料。SAAMs 的结构特点是其具有聚阳离子表面的超薄外壳，可快速激活凝血级联反应，其内部多孔葡聚糖丰富的核心可快速吸收血液和渗出物。这些特性赋予了 SAAMs 出色的止血性能，适用于急性出血。此外，由于其两亲性和阳离子表面，微胶囊内的烷基化壳聚糖具有持久的抗菌活性，可抵抗杀菌感染。此外，细胞因子可以安全地装载到无有机溶剂的微胶囊中，并精确释放，以促进表皮细胞的增殖，支持肉芽组织的后续发展，并抑制伤口愈合的最后阶段的炎症。通过制造尺寸可调的软微胶囊和实现组织修复的时间顺序功能，所提出的“全水微流控多功能生物活性 SAAMs 的生成”为伤口治疗创造了一种通用且强大的范例。
