Shi Shaochuan, Zhang Shiya, Wu Jie, Liu Xintong, Zhang Zhao
Vegetable Research Institute, Shandong Academy of Agricultural Science, Jinan, China.
Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture, China Agricultural University, Beijing, China.
Front Plant Sci. 2022 Oct 4;13:996474. doi: 10.3389/fpls.2022.996474. eCollection 2022.
Long non-coding RNAs (lncRNAs) were found to play important roles in transcriptional, post-transcriptional, and epigenetic gene regulation in various biological processes. However, lncRNAs and their regulatory roles remain poorly studied in horticultural plants. Rose is economically important not only for their wide use as garden and cut flowers but also as important sources of natural fragrance for perfume and cosmetics industry, but presently little was known about the regulatory mechanism of the floral scent production. In this paper, a RNA-Seq analysis with strand-specific libraries, was performed to rose flowers in different flowering stages. The scented variety 'Tianmidemeng' () was used as plant material. A total of 13,957 lncRNAs were identified by mining the RNA-Seq data, including 10,887 annotated lncRNAs and 3070 novel lncRNAs. Among them, 10,075 lncRNAs were predicted to possess a total of 29,622 target genes, including 54 synthase genes and 24 transcription factors related to floral scent synthesis. 425 lncRNAs were differentially expressed during the flowering process, among which 19 were differentially expressed among all the three flowering stages. Using weighted correlation network analysis (WGCNA), we correlate the differentially-expressed lncRNAs to synthesis of individual floral scent compounds. Furthermore, regulatory function of one of candidate lncRNAs for floral scent synthesis was verified using VIGS method in the rose. In this study, we were able to show that lncRNAs may play important roles in floral scent production in the rose. This study also improves our understanding of how plants regulate their secondary metabolism by lncRNAs.
长链非编码RNA（lncRNAs）被发现参与了多种生物学过程中的转录、转录后及表观遗传基因调控。然而，lncRNAs及其调控作用在园艺植物中仍未得到充分研究。玫瑰不仅作为园林花卉和切花被广泛应用，而且作为香水和化妆品行业天然香料的重要来源，具有重要的经济价值，但目前对于花香产生的调控机制知之甚少。本文对处于不同开花阶段的玫瑰花朵进行了链特异性文库的RNA测序分析。以有香味的品种‘甜蜜的梦’（）作为植物材料。通过挖掘RNA测序数据共鉴定出13957个lncRNAs，其中包括10887个注释的lncRNAs和3070个新的lncRNAs。其中，预测有10075个lncRNAs共有29622个靶基因，包括54个合成酶基因和24个与花香合成相关的转录因子。425个lncRNAs在开花过程中差异表达，其中19个在所有三个开花阶段均差异表达。使用加权基因共表达网络分析（WGCNA），我们将差异表达的lncRNAs与单个花香化合物的合成相关联。此外，利用病毒诱导的基因沉默（VIGS）方法在玫瑰中验证了一个候选花香合成lncRNAs的调控功能。在本研究中，我们能够证明lncRNAs可能在玫瑰花香产生中发挥重要作用。本研究还增进了我们对植物如何通过lncRNAs调节其次生代谢的理解。
