Guterman Inna, Shalit Moshe, Menda Naama, Piestun Dan, Dafny-Yelin Mery, Shalev Gil, Bar Einat, Davydov Olga, Ovadis Mariana, Emanuel Michal, Wang Jihong, Adam Zach, Pichersky Eran, Lewinsohn Efraim, Zamir Dani, Vainstein Alexander, Weiss David
Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food, and Environmental Quality Sciences, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel.
Plant Cell. 2002 Oct;14(10):2325-38. doi: 10.1105/tpc.005207.
For centuries, rose has been the most important crop in the floriculture industry; its economic importance also lies in the use of its petals as a source of natural fragrances. Here, we used genomics approaches to identify novel scent-related genes, using rose flowers from tetraploid scented and nonscented cultivars. An annotated petal EST database of approximately 2100 unique genes from both cultivars was created, and DNA chips were prepared and used for expression analyses of selected clones. Detailed chemical analysis of volatile composition in the two cultivars, together with the identification of secondary metabolism-related genes whose expression coincides with scent production, led to the discovery of several novel flower scent-related candidate genes. The function of some of these genes, including a germacrene D synthase, was biochemically determined using an Escherichia coli expression system. This work demonstrates the advantages of using the high-throughput approaches of genomics to detail traits of interest expressed in a cultivar-specific manner in nonmodel plants. EST sequences were submitted to the GenBank database (accession numbers BQ 103855 to BQ 106728).
几个世纪以来，玫瑰一直是花卉产业中最重要的作物；其经济重要性还体现在其花瓣可作为天然香料的来源。在此，我们利用基因组学方法，使用四倍体有香味和无香味品种的玫瑰花来鉴定与香味相关的新基因。创建了一个包含来自两个品种约2100个独特基因的花瓣EST注释数据库，并制备了DNA芯片用于所选克隆的表达分析。对两个品种挥发性成分的详细化学分析，以及对表达与香味产生一致的次生代谢相关基因的鉴定，导致发现了几个新的与花香相关的候选基因。其中一些基因的功能，包括一个吉马烯D合酶，利用大肠杆菌表达系统进行了生化测定。这项工作证明了利用基因组学的高通量方法来详细研究非模式植物中以品种特异性方式表达的感兴趣性状的优势。EST序列已提交至GenBank数据库（登录号BQ 103855至BQ 106728）。
