Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China.
Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005580.
A Gram-stain-negative, cream-coloured, aerobic, motile and ovoid- to rod-shaped bacterium, designated as FT325, was isolated from mangrove sediment collected in Shenzhen, PR China. The taxonomic position of strain FT325 was established by phylogenetic, physiological, biochemical and chemotaxonomic analyses. Strain FT325 grew optimally at 37-40 °C and pH 6.0 in the presence of 0 % (w/v) NaCl. Results of 16S rRNA gene sequence analysis showed that strain FT325 was most similarly related to CAU 1123 (96.2 %), DSM 18714 (93.9%) and HB 172011 (93.7 %). The major fatty acids (>10 %) were C 7 (60.0 %) and 11-methyl C 7 (16.7 %). The sole respiratory quinone was Q-10. The polar lipids were phosphatidylglycerol, one unidentified glycolipid, three unidentified aminolipids and three unidentified phospholipids. Its estimated genome size was 4 318 768 bp and the genomic DNA G+C content was 69.6 mol%. Based on its distinct phenotypic, chemotaxonomic and phylogenetic characteristics, strain FT325 represents a novel species of the genus , for which the name sp. nov. is proposed (=MCCC 1K07397=KCTC 92313).
一株革兰氏阴性、奶油色、需氧、运动和卵形到杆形的细菌，被命名为 FT325，从中国深圳的红树林沉积物中分离出来。通过系统发育、生理、生化和化学分类分析，确定了菌株 FT325 的分类地位。菌株 FT325 在 37-40°C 和 pH 6.0 条件下生长最佳，在 0%（w/v）NaCl 存在下。16S rRNA 基因序列分析结果表明，菌株 FT325 与 CAU 1123（96.2%）、DSM 18714（93.9%）和 HB 172011（93.7%）最为相似。主要脂肪酸（>10%）为 C7（60.0%）和 11-甲基 C7（16.7%）。唯一的呼吸醌是 Q-10。极性脂为磷脂酰甘油、一种未鉴定的糖脂、三种未鉴定的胺脂和三种未鉴定的磷脂。其估计基因组大小为 4 318 768 bp，基因组 DNA G+C 含量为 69.6 mol%。根据其独特的表型、化学分类和系统发育特征，菌株 FT325 代表了一个新的属种，建议命名为 sp. nov.（=MCCC 1K07397=KCTC 92313）。
