College of Landscape and Tourism, Hebei Agricultural University, Baoding, Hebei, China.
College of Horticulture Science & Technology, Hebei Normal University of Science & Technology, Qinhuangdao, Hebei, China.
PeerJ. 2023 Jul 19;11:e15668. doi: 10.7717/peerj.15668. eCollection 2023.
The selection and utilization of ornamental plants that are highly tolerant to salt are helpful for landscape construction and the ecological protection of coastal and arid areas. To evaluate salt tolerance, one of the most used methods is the observation of seed germination under salt stress. Therefore, this work aimed to evaluate the influence of different concentrations of NaCl in water absorption, germination, and respiratory metabolism in seeds of different genotypes. P2 and P27, salt-sensitive and salt-tolerant line s of . , were chosen for treatment with 0, 40, 80, 120, 160, 200, and 240 mM NaCl. . under salt stress exhibited inhibition of seed germination. The seeds of . have different times for the physiological phases of water absorption with different NaCl concentrations. Salt stress retarded the seed water absorption process, and it depended on seed genotypes for . . Soluble sugars accumulated in both P2 and P27 under salt stress. Meanwhile, the activities of hexokinase, 6-phosphofructokinase, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and glucose-6-phosphate dehydrogenase were overall increased in P27 after salt treatment, which caused increases in pyruvic acid and citric acid. The citrate synthase and glucose-6-phosphate dehydrogenase activities decreased in P2. These results suggest that the respiratory metabolism of salt-tolerant . was enhanced, compared with the salt-sensitive line, to ameliorate the repression of seed germination under salt stress. The different changes in respiratory metabolism could influence the degree of salt tolerance.
选择和利用对盐高度耐受的观赏植物有助于景观建设和沿海及干旱地区的生态保护。为了评估盐度耐受性，最常用的方法之一是观察盐胁迫下种子的萌发。因此，本工作旨在评估不同浓度 NaCl 对不同基因型种子吸水、萌发和呼吸代谢的影响。选择盐敏感和盐耐受系 P2 和 P27 进行 0、40、80、120、160、200 和 240mM NaCl 处理。盐胁迫下种子萌发受到抑制。不同基因型种子在不同 NaCl 浓度下的吸水生理阶段时间不同。盐胁迫延缓了种子的吸水过程，这取决于种子基因型。盐胁迫下 P2 和 P27 中的可溶性糖积累。同时，盐处理后 P27 中的己糖激酶、6-磷酸果糖激酶、丙酮酸激酶、丙酮酸脱氢酶、柠檬酸合酶和葡萄糖-6-磷酸脱氢酶的活性总体增加，导致丙酮酸和柠檬酸增加。P2 中的柠檬酸合酶和葡萄糖-6-磷酸脱氢酶活性降低。这些结果表明，与盐敏感系相比，耐盐系的呼吸代谢增强，以改善盐胁迫下种子萌发的抑制。呼吸代谢的不同变化可能会影响盐度耐受性。
