血清活性 B12（全钴胺素）在真正评估维生素 B12 缺乏症中的诊断可靠性：当前视角的相关性。

Diagnostic reliability of serum active B12 (holo-transcobalamin) in true evaluation of vitamin B12 deficiency: Relevance in current perspective.

机构信息

Department of Biochemistry, Ramakrishna Mission Seva Pratishthan (RKMSP) hospital, 700026, Kolkata, West Bengal, India.

Department of Biomedical Science and Technology, School of Biological Sciences, JIVAN, Ramakrishna Mission Vivekananda Educational and Research Institute (RKMVERI), 700026, Kolkata, West Bengal, India.

出版信息

BMC Res Notes. 2022 Oct 22;15(1):329. doi: 10.1186/s13104-022-06224-8.

DOI:10.1186/s13104-022-06224-8

PMID:36273211

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9588217/

Abstract

OBJECTIVE

Measurement of total vitamin B12 (vit B12) concentration raised concerns over early detection of vit B12 deficiency due to its clinical unreliability. In this present article we aimed to assess the efficacy of holo-transcobolamin (active vit B12) for true evaluation of vit B12 deficiency.

METHODS

This retrospective study included 100 participants referred for vit B12 assay. Serum total vit B12, active vit B12 and homocysteine were estimated.

RESULTS

Our study showed 59% of the total participants with vit B12 deficiency (185 ± 64.62 pg/ml) and 18% with hyper-cobalaminemia (1666.9 ± 367.13 pg/ml) based on their total vit B12 concentrations. A comparative study on total vit B12 and active vit B12 was done which reflected a striking disparity in results. Active vit B12 reported 28.8% patients with vit B12 deficiency (19.8 ± 17.48 pg/ml) and only 16.6% patients with hyper-cobalaminemia (224.14 ± 10 pg/ml). Active vit B12 appeared to be more sensitive (82.35% vs 65%) and specific (46.6% vs. 43.8%) diagnostic marker compared to total vit B12. Pearson Correlation study indicated a strong positive correlation (r = 0.695 at p < 0.01) hence justified use of the two methods.

CONCLUSION

We claim that active vit B12 is a much more reliable biomarker than total vit B12 for early diagnosis of vit B12 deficiency.

摘要

目的

总维生素 B12（vit B12）浓度的测量引起了人们对早期发现维生素 B12 缺乏的关注，因为其临床不可靠。在本文中，我们旨在评估全钴胺素（活性 vit B12）对维生素 B12 缺乏的真实评估的功效。

方法

这项回顾性研究包括 100 名接受维生素 B12 检测的参与者。估计血清总维生素 B12、活性维生素 B12 和同型半胱氨酸。

结果

我们的研究显示，根据总维生素 B12 浓度，59%的总参与者存在维生素 B12 缺乏症（185±64.62 pg/ml），18%存在高钴胺血症（1666.9±367.13 pg/ml）。对总维生素 B12 和活性维生素 B12 进行了比较研究，结果显示结果存在显著差异。活性维生素 B12 报告有 28.8%的维生素 B12 缺乏症患者（19.8±17.48 pg/ml），只有 16.6%的高钴胺血症患者（224.14±10 pg/ml）。与总维生素 B12 相比，活性维生素 B12 似乎是更敏感（82.35% vs 65%）和特异（46.6% vs. 43.8%）的诊断标志物。Pearson 相关研究表明，两者之间存在很强的正相关（r=0.695，p<0.01），因此可以合理地使用这两种方法。