van Schothorst M, Gilbert R J, Harvey R W, Pietzsch O, Kampelmacher E H
Zentralbl Bakteriol B. 1978 Aug;167(1-2):138-45.
Comparative studies carried out in four laboratories revealed that in the examination of frozen or fresh minced meat samples tryptone soya broth and glucose mineral salts medium used for pre-enrichment gave the same results as buffered peptone water. The three tetrathionate media (Cardiff formula, Difco and Oxoid) used for enrichment after pre-enrichment, yielded approximately the same number of positive samples and about the same number of frozen and cooled samples was found positive when data of all four laboratories were considered. Between laboratories a considerable variation in results was found. This indicates the necessity to conduct comparative trials in more than one laboratory to validate the use of a specific method for regulatory purposes.
在四个实验室进行的对比研究表明,在检测冷冻或新鲜碎肉样本时,用于预富集的胰蛋白胨大豆肉汤和葡萄糖矿物质盐培养基与缓冲蛋白胨水的检测结果相同。预富集后用于富集的三种四硫磺酸盐培养基(加的夫配方、迪夫科和奥克托)产生的阳性样本数量大致相同,当综合四个实验室的数据时,发现冷冻和冷藏样本中呈阳性的数量也大致相同。不同实验室之间的结果存在相当大的差异。这表明有必要在多个实验室进行对比试验,以验证特定方法在监管目的上的适用性。