Siems H
Zentralbl Bakteriol B. 1978 Aug;167(1-2):120-8.
146 samples of pancreatic powder, 208 samples of pasteurized egg albumen, both deep-frozen and spray-dried, and 355 samples of frozen poultry were investigated for the presence of salmonellae. Four pre-enrichment media (lactose broth, nutrient broth, buffered peptone water and tryptone soya broth) and two direct selective enrichment media (dulcitol selenite and tetrathionate) were used. For the pre-enrichment of frozen poultry, buffered peptone water and tryptone soya broth proved to be more satisfactory than lactose broth and nutrient broth. Pre-enrichment of freeze-dried material in a non-selective medium yielded more Salmonella than were obtained by a direct selective enrichment. 16 of 146 samples of freeze-dried pancreatic powder contained salmonellae. Accordingly, tissue and organs of slaughter animals designed for the production of pharmaceutical preparations require regular bacteriological control.
对146份胰粉样本、208份经过巴氏杀菌的冷冻和喷雾干燥蛋清样本以及355份冷冻家禽样本进行了沙门氏菌检测。使用了四种预增菌培养基(乳糖肉汤、营养肉汤、缓冲蛋白胨水和胰蛋白胨大豆肉汤)和两种直接选择性增菌培养基(亚硒酸盐山梨醇和四硫磺酸盐)。对于冷冻家禽的预增菌,缓冲蛋白胨水和胰蛋白胨大豆肉汤比乳糖肉汤和营养肉汤更令人满意。在非选择性培养基中对冻干材料进行预增菌比直接选择性增菌获得的沙门氏菌更多。146份冻干胰粉样本中有16份含有沙门氏菌。因此,用于生产药物制剂的屠宰动物的组织和器官需要定期进行细菌学控制。