Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al Hofuf, Al-Ahsa, 31982, Saudi Arabia.
Department of Internal Medicine, Infectious Diseases and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Trop Anim Health Prod. 2022 Oct 25;54(6):361. doi: 10.1007/s11250-022-03356-8.
Uterine diseases are prevalent in camels and lead to economic losses because of decreased fertility. The aim of this study is to look into the expression patterns of acute-phase proteins (APPs) and inflammatory cytokines in dromedary camels with clinical endometritis (CE) to highlight their role in the immune pathogenesis of the disease. Moreover, to identify the use of these parameters as a complementary tool for CE screening as well as investigate the efficacy of ceftiofur antibiotic, APPs and inflammatory cytokines were estimated in camels with CE. Values of APPs (Hp, SAA, and Fg), pro-inflammatory (IFN-γ, TNF-α, IL-1α, IL-1β, and IL-6), and anti-inflammatory cytokines (IL-10) were higher in camels with CE than in healthy controls (P < 0.05). The strongest correlations were observed between HP and IFN-γ (r = 0.73) and IL-1β and IL-6 (r = 0.73), while the weakest correlations were observed between Fg and IFN-γ (r = 0.25). Corynebacterium pyogenes and Arcanobacterium pyogenes were the most common pathogens involved in the etiology of CE. All investigated biomarkers demonstrated a high degree of recognition between CE camel and healthy controls (AUC was > 0.90). A higher proportion of camels with CE that were treated with ceftiofur (90%, P < 0.0001) scored clinical cures after the first dose, while 10% required a second dose. In conclusion, CE causes increased APPs and inflammatory cytokine biomarkers, indicating a significant acute phase response in diseased camels with CE. These changes in biomarkers could be beneficial for understanding the immune pathogenesis of CE in dromedary camels, clinical practice, and basic clinical research.
子宫疾病在骆驼中很常见，导致生育力下降，造成经济损失。本研究旨在研究临床子宫内膜炎（CE）中骆驼急性期蛋白（APPs）和炎症细胞因子的表达模式，以突出它们在疾病免疫发病机制中的作用。此外，为了确定这些参数作为 CE 筛查的补充工具的用途，并研究头孢噻呋抗生素的疗效，在患有 CE 的骆驼中评估了 APPs 和炎症细胞因子。患有 CE 的骆驼的 APPs（Hp、SAA 和 Fg）、促炎细胞因子（IFN-γ、TNF-α、IL-1α、IL-1β 和 IL-6）和抗炎细胞因子（IL-10）的值高于健康对照组（P<0.05）。在患有 CE 的骆驼中观察到 HP 与 IFN-γ（r=0.73）和 IL-1β 与 IL-6（r=0.73）之间的相关性最强，而 Fg 与 IFN-γ（r=0.25）之间的相关性最弱。引起 CE 的最常见病原体是化脓隐秘杆菌和隐秘螺旋体。所有研究的生物标志物在 CE 骆驼和健康对照组之间均表现出高度的识别能力（AUC>0.90）。接受头孢噻呋治疗的 CE 骆驼中，有更高比例（90%，P<0.0001）在第一剂后即获得临床治愈，而有 10%需要第二剂。总之，CE 导致 APPs 和炎症细胞因子生物标志物增加，表明患有 CE 的骆驼出现明显的急性期反应。这些生物标志物的变化可能有助于了解 CE 在单峰驼中的免疫发病机制、临床实践和基础临床研究。
