sp. nov., isolated from soil in Malaysia.

A urease-producing Gram-stain-positive actinobacterium, designated strain T5, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia. The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar. Strain T5 grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl. The whole-cell sugar hydrolysate of strain T5 contained galactose, glucose and ribose. The ll-diaminopimelic acid isomer was detected in the cell wall. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids. The main fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. Comparative analysis of the 16S rRNA gene sequences indicated that strain T5 belonged to of the family with the highest 16S rRNA gene sequence similarity to LCR6-01 (99.0 %). The overall genome relatedness indices revealed that the closest related species was LCR6-01 with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization. Phylogeny analyses showed that strain T5 was closely related to , , , and . Based on these polyphasic data, strain T5 represents a novel species, for which the name sp. nov. is proposed. The type strain is T5 (=TBRC 5137= DSM 42166).