Kanchanasin Pawina, Sripreechasak Paranee, Suriyachadkun Chanwit, Supong Khomsan, Pittayakhajonwut Pattama, Somphong Achiraya, Tanasupawat Somboon, Phongsopitanun Wongsakorn
Department of Microbiology, Faculty of Science, Burapha University, Chonburi 20131, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005682.
A novel actinomycete, strain RY43-2, belonging to the genus , was isolated from a peat swamp forest soil collected from Rayong Province, Thailand. The strain was characterized by using a polyphasic approach. The cell-wall peptidoglycan contained ll-diaminopimelic. Ribose and glucose were detected in its whole-cell hydrolysates. The strain contained anteiso-C, iso-C and iso-C as the predominant fatty acids, and MK-9(H), MK-9(H) and MK-9(H) as the major menaquinones. The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, three unidentified ninhydrin-positive phospholipids and two unidentified phospholipids. Strain RY43-2 showed the highest 16S rRNA gene similarity to JCM 4497 (98.9 %) and LCR6-01 (98.9 %). The draft genome of RY43-2 was 6.7 Mb with 6078 coding sequences with an average G+C content of 70.8 mol%. Genomic analysis revealed that the average nucleotide identity (ANI) values based on blast (ANIb) and MUMmer (ANIm) between strain RY43-2 and JCM 4497 were 80.1 and 86.1%, respectively. The ANIb and ANIm values between strain RY43-2 and LCR6-01 were 77.0 and 85.5%, respectively. The digital DNA-DNA hybridization values were 25.2 and 23.0% in comparison with the draft genomes of JCM 4497 and LCR6-01, respectively. The results of taxonomic analysis suggested that strain RY43-2 represented a novel species of the genus for which the name sp. nov. is proposed. The type strain is RY43-2 (=TBRC 7286=NBRC 115640). Strain RY43-2 exhibited antimicrobial activity against ATCC 51559, BMGC 106 and BMGC 107 with the minimum inhibitory concentration values of 25.0, 12.5, and 6.25 µg ml. It also exhibited potent antimalarial activity against K1 with IC of 0.0031 µg ml. In addition, it showed cytotoxicity against Vero, KB, MCF-7 and NCI-H187 with IC values of 0.0347, 6.15, 3.36 and 0.0352 µg ml, respectively.
从泰国罗勇府采集的泥炭沼泽森林土壤中分离出一种新型放线菌，菌株RY43 - 2，属于 属。采用多相分类法对该菌株进行了表征。细胞壁肽聚糖含有ll - 二氨基庚二酸。在其全细胞水解产物中检测到核糖和葡萄糖。该菌株以anteiso - C、iso - C和iso - C作为主要脂肪酸，以MK - 9(H)、MK - 9(H)和MK - 9(H)作为主要甲基萘醌。磷脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、三种未鉴定的茚三酮阳性磷脂和两种未鉴定的磷脂。菌株RY43 - 2与JCM 4497（98.9%）和LCR6 - 01（98.9%）的16S rRNA基因相似度最高。RY43 - 2的基因组草图为6.7 Mb，有6078个编码序列，平均G + C含量为70.8 mol%。基因组分析表明，基于blast（ANIb）和MUMmer（ANIm），菌株RY43 - 2与JCM 4497之间的平均核苷酸同一性（ANI）值分别为80.1%和86.1%。菌株RY43 - 2与LCR6 - 01之间的ANIb和ANIm值分别为77.0%和85.5%。与JCM 4497和LCR6 - 01的基因组草图相比，数字DNA - DNA杂交值分别为25.2%和23.0%。分类分析结果表明，菌株RY43 - 2代表 属的一个新物种，为此提出新名称 sp. nov.。模式菌株为RY43 - 2（=TBRC 7286=NBRC 115640）。菌株RY43 - 2对ATCC 51559、BMGC 106和BMGC 107表现出抗菌活性，最低抑菌浓度值分别为25.0、12.5和6.25 µg/ml。它对K1也表现出强效抗疟活性，IC为0.0031 µg/ml。此外，它对Vero、KB、MCF - 7和NCI - H187表现出细胞毒性，IC值分别为0.0347、6.15、3.36和0.0352 µg/ml。
