Chambers Emma S, Vukmanovic-Stejic Milica, Turner Carolin T, Shih Barbara B, Trahair Hugh, Pollara Gabriele, Tsaliki Evdokia, Rustin Malcolm, Freeman Tom C, Mabbott Neil A, Noursadeghi Mahdad, Martineau Adrian R, Akbar Arne N
Centre for Immunobiology, Blizard Institute, Queen Mary University of London, London, UK.
Division of Infection and Immunity, University College London, London, UK.
Immunother Adv. 2020 Nov 25;1(1):ltaa008. doi: 10.1093/immadv/ltaa008. eCollection 2021 Jan.
Ageing is associated with increased number of infections, decreased vaccine efficacy and increased systemic inflammation termed inflammageing. These changes are reflected by reduced recall responses to varicella zoster virus (VZV) challenge in the skin of older adults. Vitamin D deficiency is more common in the old and has been associated with frailty and increased inflammation. In addition, vitamin D increases immunoregulatory mechanisms and therefore has the potential to inhibit inflammageing.
We investigated the use of vitamin D replacement to enhance cutaneous antigen-specific immunity in older adults (≥65 years).
Vitamin D insufficient older adults ( = 18) were administered 6400IU of vitamin D/day orally for 14 weeks. Antigen-specific immunity to VZV was assessed by clinical score assessment of the injection site and transcriptional analysis of skin biopsies collected from challenged injection sites pre- and post-vitamin D replacement.
We showed that older adults had reduced VZV-specific cutaneous immune response and increased non-specific inflammation as compared to young. Increased non-specific inflammation observed in the skin of older adults negatively correlated with vitamin D sufficiency. We showed that vitamin D supplementation significantly increased the response to cutaneous VZV antigen challenge in older adults. This enhancement was associated with a reduction in inflammatory monocyte infiltration with a concomitant enhancement of T cell recruitment to the site of antigen challenge in the skin.
Vitamin D replacement can boost antigen-specific immunity in older adults with sub-optimal vitamin D status.
衰老与感染次数增加、疫苗效力下降以及称为炎症衰老的全身炎症增加有关。这些变化表现为老年人皮肤对水痘带状疱疹病毒（VZV）攻击的回忆反应减弱。维生素D缺乏在老年人中更为常见，并且与身体虚弱和炎症增加有关。此外，维生素D可增强免疫调节机制，因此具有抑制炎症衰老的潜力。
我们研究了补充维生素D对增强老年人（≥65岁）皮肤抗原特异性免疫力的作用。
对18名维生素D不足的老年人每天口服6400IU维生素D，持续14周。通过对注射部位进行临床评分评估以及对维生素D补充前后从受攻击的注射部位采集的皮肤活检组织进行转录分析，来评估对VZV的抗原特异性免疫力。
我们发现，与年轻人相比，老年人的VZV特异性皮肤免疫反应减弱，非特异性炎症增加。在老年人皮肤中观察到的非特异性炎症增加与维生素D充足程度呈负相关。我们发现补充维生素D可显著增强老年人对皮肤VZV抗原攻击的反应。这种增强与炎症单核细胞浸润减少相关，同时T细胞向皮肤抗原攻击部位的募集增强。
补充维生素D可增强维生素D水平欠佳的老年人的抗原特异性免疫力。
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