Mehwish Nabila, Xu Mengdie, Zaeem Muhammad, Lee Bae Hoon
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325011, China.
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325000, China.
Gels. 2022 Oct 20;8(10):679. doi: 10.3390/gels8100679.
A crucial method for adding new functions to current biomaterials for biomedical applications has been surface functionalization via molecular design. Mussel-inspired polydopamine (PDA) has generated much attention as a facile method for the functionalization of biomaterials because of its substantial independence in deposition, beneficial cell interactions, and significant responsiveness aimed at secondary functionalization. Because of their porous structure, the bovine serum albumin methacryloyl (BSAMA)-BM cryogels were functionalized with PDA (BM-PDA), which may reproduce the architecture and biological purpose of the natural extracellular environment. Excellent antioxidative and antibacterial qualities, improved mineralization, and better cell responsiveness were all demonstrated by BM-PDA. BM-PDA scaffolds maintained their linked and uniform pores after functionalization, which can make it easier for nutrients to be transported during bone repair. As a result, hydroxyapatite (HA)-coated BM* and BM-PDA* cryogels were created through successive mineralization with the goal of mineralized bone tissue repair. The heterogeneous nucleation and surface roughness contributed to rod-like apatite production in BM-PDA* cryogels whereas BM* cryogels were made up of plate-like HA morphologies. Analysis results showed that after five cycles, the mineral contents were around 57% and the HA units remained equally dispersed on the surface of BM-PDA* with a Ca/P ratio of 1.63. Other natural polymer-based cryogels can be coated using this general, rapid, and simple PDA coating technique and utilized as implants for bone tissue engineering. Future clinical uses of albumin cryogels for bone tissue engineering will advance as a result of additional in-vivo testing of such PDA-coated cryogels.
通过分子设计进行表面功能化是一种向当前用于生物医学应用的生物材料添加新功能的关键方法。受贻贝启发的聚多巴胺（PDA）作为一种生物材料功能化的简便方法备受关注，因为它在沉积方面具有很大的独立性、有益的细胞相互作用以及针对二次功能化的显著响应性。由于其多孔结构，用PDA对甲基丙烯酰化牛血清白蛋白（BSAMA）-BM冷冻凝胶进行功能化（BM-PDA），这可以重现天然细胞外环境的结构和生物学功能。BM-PDA表现出优异的抗氧化和抗菌性能、改善的矿化作用以及更好的细胞反应性。功能化后，BM-PDA支架保持其连接且均匀的孔隙，这有助于在骨修复过程中营养物质的运输。因此，通过连续矿化制备了羟基磷灰石（HA）涂层的BM和BM-PDA冷冻凝胶，旨在修复矿化骨组织。异质成核和表面粗糙度有助于在BM-PDA冷冻凝胶中生成棒状磷灰石，而BM冷冻凝胶则由板状HA形态组成。分析结果表明，经过五个循环后，矿化含量约为57%，HA单元在BM-PDA*表面均匀分散，钙磷比为1.63。这种通用、快速且简单的PDA涂层技术可用于涂覆其他基于天然聚合物的冷冻凝胶，并用作骨组织工程的植入物。对这种PDA涂层冷冻凝胶进行更多的体内测试将推动白蛋白冷冻凝胶在骨组织工程中的未来临床应用。
