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基体在纤毛力的作用下弯曲。

Basal bodies bend in response to ciliary forces.

机构信息

Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045.

Department of Mechanical Engineering and Material Science, Washington University in St. Louis, St. Louis, MO 63130.

出版信息

Mol Biol Cell. 2022 Dec 1;33(14):ar146. doi: 10.1091/mbc.E22-10-0468-T. Epub 2022 Oct 26.

DOI:10.1091/mbc.E22-10-0468-T
PMID:36287828
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9727800/
Abstract

Motile cilia beat with an asymmetric waveform consisting of a power stroke that generates a propulsive force and a recovery stroke that returns the cilium back to the start. Cilia are anchored to the cell cortex by basal bodies (BBs) that are directly coupled to the ciliary doublet microtubules (MTs). We find that, consistent with ciliary forces imposing on BBs, bending patterns in BB triplet MTs are responsive to ciliary beating. BB bending varies as environmental conditions change the ciliary waveform. Bending occurs where striated fibers (SFs) attach to BBs and mutants with short SFs that fail to connect to adjacent BBs exhibit abnormal BB bending, supporting a model in which SFs couple ciliary forces between BBs. Finally, loss of the BB stability protein Poc1, which helps interconnect BB triplet MTs, prevents the normal distributed BB and ciliary bending patterns. Collectively, BBs experience ciliary forces and manage mechanical coupling of these forces to their surrounding cellular architecture for normal ciliary beating.

摘要

纤毛以不对称的波形运动，该波形由一个产生推进力的动力冲程和一个将纤毛返回到起始位置的恢复冲程组成。纤毛通过基底体 (BB) 锚定在细胞皮层上，基底体直接与纤毛二联体微管 (MT) 相连。我们发现，与纤毛力对 BB 的施加一致，BB 三联体 MT 的弯曲模式对纤毛的拍打有反应。随着环境条件改变纤毛波形，弯曲发生变化。在条纹纤维 (SF) 附着到 BB 的地方发生弯曲，并且具有短 SF 而未能连接到相邻 BB 的突变体表现出异常的 BB 弯曲，支持 SF 将纤毛力在 BB 之间连接的模型。最后，失去有助于 BB 三联体 MT 互连的 BB 稳定性蛋白 Poc1 会阻止正常分布的 BB 和纤毛弯曲模式。总的来说，BB 会经历纤毛力，并管理这些力与其周围细胞结构的机械耦合，以实现正常的纤毛拍打。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/0c33761fd60e/mbc-33-ar146-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/2ee00ff26032/mbc-33-ar146-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/3ac309021dc1/mbc-33-ar146-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/cbfc4f2cfbf7/mbc-33-ar146-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/263fab5bee92/mbc-33-ar146-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/e3ea28baa719/mbc-33-ar146-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/0c33761fd60e/mbc-33-ar146-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/2ee00ff26032/mbc-33-ar146-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/3ac309021dc1/mbc-33-ar146-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/cbfc4f2cfbf7/mbc-33-ar146-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/263fab5bee92/mbc-33-ar146-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/e3ea28baa719/mbc-33-ar146-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5f3/9727800/0c33761fd60e/mbc-33-ar146-g006.jpg

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