Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1, Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Advanced Genomics Center, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005596.
Two strictly anaerobic, Gram-stain-positive, non-motile bacteria (strains OPF53 and TOC12) were isolated from mouse intestines. Strains OPF53 and TOC12 grew at pH 5.5-9.0 and 5.0-9.0, respectively, and at temperatures of 30-45 °C. The cell morphologies of these strains were short rods and rods, respectively, and the cells possessed intracellular granules. The major cellular fatty acids of OPF53 were C 9 and C 9 dimethyl acetal, whereas those of TOC12 were C and C 9. In OPF53, the main end-products of modified peptone-yeast extract-glucose (PYG) fermentation were lactate, formate and butyrate, whereas, in addition to these acids, TOC12 also produced hydrogen. The genomes of OPF53 and TOC12 were respectively 2.2 and 2.0 Mbp in size with a DNA G+C contents of 69.1 and 58.7 %. The 16S rRNA gene sequences of OPF53 and TOC12 showed the highest similarity to members of the family , namely, Marseille-P2936 (94.3 %) and KCTC 15140 (93.2 %), respectively. Phylogenetic analyses revealed that both isolates formed distinct lineages from other genera of the family . In addition, the two strains were characterized by relatively low 16S rRNA gene sequence similarity (93.4 %) and can be distinguished by their distinctive traits (including cell shape, DNA G+C content, and major fatty acids profiles). On the basis of their polyphasic taxonomic properties, these isolates represent two noel species of two novel genera within the family , for which the names gen. nov., sp. nov. (OPF53=JCM 35015=KCTC 25474) and gen. nov., sp. nov. (TOC12=JCM 35017=KCTC 25472) are proposed.
从鼠肠中分离到两株严格厌氧、革兰氏阳性、不运动的细菌（OPF53 株和 TOC12 株）。OPF53 株和 TOC12 株分别在 pH5.5-9.0 和 5.0-9.0 以及 30-45°C 的温度下生长。这两种菌株的细胞形态分别为短杆菌和杆菌，细胞内都含有颗粒。OPF53 株的主要细胞脂肪酸为 C9 和 C9 二甲缩醛，而 TOC12 株的主要细胞脂肪酸为 C16:0 和 C18:1ω9c。在 OPF53 株中，改良的蛋白胨-酵母提取物-葡萄糖（PYG）发酵的主要末端产物为乳酸盐、甲酸盐和丁酸盐，而 TOC12 株除了这些酸之外，还产生氢气。OPF53 株和 TOC12 株的基因组大小分别为 2.2 和 2.0 Mbp，DNA G+C 含量分别为 69.1%和 58.7%。OPF53 株和 TOC12 株的 16S rRNA 基因序列与马赛 P2936 属（94.3%）和 KCTC 15140 属（93.2%）的成员最为相似。系统发育分析表明，这两个分离株均与该科的其他属形成明显不同的进化枝。此外，这两个菌株的 16S rRNA 基因序列相似性较低（93.4%），并且可以通过其独特的特征（包括细胞形状、DNA G+C 含量和主要脂肪酸图谱）来区分。基于多相分类学特性，这两个分离株代表了该科的两个新属中的两个新种，分别命名为 gen. nov.，sp. nov.（OPF53=JCM 35015=KCTC 25474）和 gen. nov.，sp. nov.（TOC12=JCM 35017=KCTC 25472）。
