ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Vitabio, Inc., Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006382.
Two rod-shaped, obligate anaerobic, Gram-stain-positive bacteria isolated from the pig faeces were designated YH-ols2216 and YH-ols2217. Analysis of 16S rRNA gene sequences revealed that these isolates were most related to the members of the family , within the order , and KCTC 25474 with 92.0 and 92.5% similarities, respectively. The 16S rRNA gene sequence similarity within isolates was 99.9 %; and those between isolates YH-ols2216 and YH-ols2217, and DSM 20586, the type species of the type genus within the family , were 88.5 and 88.7 %, respectively. Those between isolates and PW2, the type species of the type genus within the family , were 88.7 and 89.1 %, respectively. The multi-locus sequence tree revealed that the isolates, alongside the genera and , formed a distinct cluster between the families and . The average nucleotide identities and digital DNA-DNA hybridization values for the isolates and their most closely related strains ranged from 67.7 to 76.2 % and from 18.4 to 23.3 %, respectively. The main cellular fatty acids of the isolates were C DMA, C 9, C 12OH, C, and C. The cell wall contained the peptidoglycan -diaminopimelic acid. Lactate was the main end-product of the isolates. The major polar lipids of isolate YH-ols2217 were aminophospholipid, aminolipids, and lipids. Menaquinones were not identified in the cells of the isolates. The DNA G+C contents of isolates YH-ols2216 and YH-ols2217 were 67.5 and 67.6 mol%, respectively. Considering these chemotaxonomic, phenotypic, and phylogenetic properties, fam. nov. is proposed within the order . YH-ols2216 (=KCTC 25708=NBRC 116429) and YH-ols2217 (=KCTC 25709=NBRC 116430) represent a novel taxon within this new family and the name gen. nov., sp. nov. is proposed. In addition, the genera and are transferred to the family fam. nov.
从猪粪便中分离到两株杆状、严格厌氧菌、革兰氏阳性的细菌，命名为 YH-ols2216 和 YH-ols2217。16S rRNA 基因序列分析表明，这两株菌与 目内的 科、 属的成员最为密切相关，与 KCTC 25474 的相似度分别为 92.0%和 92.5%。两株菌的 16S rRNA 基因序列相似度为 99.9%；与 YH-ols2216 和 YH-ols2217 分离株之间的相似度分别为 88.5%和 88.7%，与 科的模式种 DSM 20586 的相似度分别为 88.5%和 88.7%。与 科的模式种 PW2 的相似度分别为 88.7%和 89.1%。多位点序列树显示，这些分离株与 和 属一起，在 科和 科之间形成了一个独特的聚类。分离株与其最密切相关菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 67.7%至 76.2%和 18.4%至 23.3%。分离株的主要细胞脂肪酸为 CDMA、C9、C12OH、C14:0 和 C16:0。细胞壁含有肽聚糖-二氨基庚二酸。乳酸是分离株的主要终产物。分离株 YH-ols2217 的主要极性脂为氨基磷脂、氨基脂和脂类。分离株细胞中未鉴定出menaquinones。分离株 YH-ols2216 和 YH-ols2217 的 DNA G+C 含量分别为 67.5mol%和 67.6mol%。考虑到这些化学分类学、表型和系统发育特征，提议在 目中建立新的科 fam. nov.。YH-ols2216（=KCTC 25708=NBRC 116429）和 YH-ols2217（=KCTC 25709=NBRC 116430）代表这个新科内的一个新分类群，提议使用 gen. nov.，sp. nov.作为新种名。此外， 和 属被转移到新科 fam. nov.。
