Li Xiaoqiong, Jensen Rikke Lassen, Højberg Ole, Canibe Nuria, Jensen Bent Borg
Department of Animal Science, Aarhus University, Tjele, Denmark.
Department of Large Animal Science, University of Copenhagen, Copenhagen, Denmark.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1227-1233. doi: 10.1099/ijs.0.000083. Epub 2015 Jan 29.
Strain SK9K4(T), which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4(T) metabolized indol-3-acetic acid to 3-methylindole (skatole), which is the main contributor to boar taint; it also produced 4-methylphenol (p-cresol) from p-hydroxyphenylacetic acid. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that the isolate represented a new lineage within the genus Olsenella of the family Atopobiaceae . Strain SK9K4(T) was most closely related to the type strains of the three species of the genus Olsenella with validly published names; Olsenella profusa DSM 13989(T) (93.6%), Olsenella uli DSM 7084(T) (93.5%) and Olsenella umbonata DSM 22620(T) (92.7%). DNA-DNA relatedness values of strain SK9K4(T) with O. profusa , O. uli and O. umbonata were 28.3%, 69.1% and 27.2%, respectively. The genomic DNA G+C content was 62.1 mol% and the major cellular fatty acids (constituting >10% of the total) were C(14 : 0) and C(18 : 1)ω9c. The major end product of glucose fermentation was lactic acid, with minor amounts of acetic acid and formic acid; no H2 was produced. Discrepancies in the fatty acid profiles, the MALDI-TOF mass spectra of cell extracts and the physiological and biochemical characteristics differentiated strain SK9K4(T) from other species of the genus Olsenella and indicate that the isolate represents a novel species within this genus. The name Olsenella scatoligenes sp. nov., is proposed and the type strain is SK9K4(T) ( = JCM 19907(T) = DSM 28304(T)).
菌株SK9K4(T)是从猪粪便中分离得到的，它是一种严格厌氧、无运动性、不产芽孢、革兰氏染色阳性、能分解糖类的球杆菌。SK9K4(T)可将吲哚-3-乙酸代谢为3-甲基吲哚（粪臭素），而粪臭素是公猪异味的主要成因；它还能从对羟基苯乙酸产生4-甲基苯酚（对甲酚）。基于16S rRNA基因序列的系统发育分析表明，该分离株代表了阿托波氏菌科奥尔森氏菌属内的一个新谱系。菌株SK9K4(T)与奥尔森氏菌属三个有效发表名称的种的模式菌株关系最为密切；即丰富奥尔森氏菌DSM 13989(T)（93.6%）、乌利奥尔森氏菌DSM 7084(T)（93.5%）和瘤突奥尔森氏菌DSM 22620(T)（92.7%）。菌株SK9K4(T)与丰富奥尔森氏菌、乌利奥尔森氏菌和瘤突奥尔森氏菌的DNA-DNA相关性值分别为28.3%、69.1%和27.2%。基因组DNA的G+C含量为62.1 mol%，主要细胞脂肪酸（占总量>10%）为C(14 : 0)和C(18 : 1)ω9c。葡萄糖发酵的主要终产物是乳酸，还有少量乙酸和甲酸；不产生H2。脂肪酸谱、细胞提取物的基质辅助激光解吸电离飞行时间质谱以及生理生化特性的差异将菌株SK9K4(T)与奥尔森氏菌属的其他种区分开来，表明该分离株代表了该属内的一个新种。提议将其命名为产粪臭素奥尔森氏菌（Olsenella scatoligenes）新种，模式菌株为SK9K4(T)（=JCM 19907(T)=DSM 28304(T)）。
