Kopcakova Anna, Salamunova Slavomira, Javorsky Peter, Sabo Rastislav, Legath Jaroslav, Ivorova Silvia, Piknova Maria, Pristas Peter
Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Soltesovej 4-6, 040 01 Kosice, Slovakia.
Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovakia.
Vet Sci. 2022 Sep 23;9(10):521. doi: 10.3390/vetsci9100521.
In recent decades, the significant deterioration of the health status of honey bees has been observed throughout the world. One of the most severe factors affecting the health of bee colonies worldwide is American foulbrood disease. This devastating disease, with no known cure, is caused by the Gram-positive spore-forming bacteria of species. At present, DNA-based methods are being used for identification and typing. In our study, we compare two of the most advanced DNA-based technologies (rep-PCR and 16S rRNA analyses) with MALDI-TOF MS fingerprinting to evaluate variability in Central Europe. While 16S rRNA analysis presents a very limited variation among the strains, MALDI-TOF MS is observed to be more efficient at differentiating . Remarkably, no clear correlation is observed between whole-genome rep-PCR fingerprinting and MALDI-TOF MS-based typing. Our data indicate that MALDI-TOF protein profiling provides accurate and cost-effective methods for the rapid identification of strains and provides novel perspectives on strain diversity compared to conventional DNA-based genotyping approaches. The current study provides a good foundation for future studies.
近几十年来，全世界都观察到蜜蜂健康状况的显著恶化。影响全球蜂群健康的最严重因素之一是美洲幼虫腐臭病。这种毁灭性疾病尚无已知治愈方法，由特定的革兰氏阳性产芽孢细菌引起。目前，基于DNA的方法正用于鉴定和分型。在我们的研究中，我们将两种最先进的基于DNA的技术（重复序列PCR和16S rRNA分析）与基质辅助激光解吸电离飞行时间质谱指纹图谱法进行比较，以评估中欧地区的变异性。虽然16S rRNA分析显示菌株间变异非常有限，但观察到基质辅助激光解吸电离飞行时间质谱在区分菌株方面更有效。值得注意的是，全基因组重复序列PCR指纹图谱法与基于基质辅助激光解吸电离飞行时间质谱的分型之间未观察到明显相关性。我们的数据表明，基质辅助激光解吸电离飞行时间蛋白质分析提供了准确且经济高效的方法来快速鉴定菌株，并且与传统的基于DNA的基因分型方法相比，为菌株多样性提供了新的视角。当前的研究为未来的研究奠定了良好基础。
