Chaprazov Tsvetan, Vasileva Radina, Atliev Kiril, Firkova Elena
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.
Department of Urology and General Medicine Medical, Faculty of Medicine, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria.
Vet Sci. 2022 Oct 18;9(10):578. doi: 10.3390/vetsci9100578.
The purpose of this study was to compare the effects of local and systemic application of recombinant human erythropoietin (rhEPO) on the healing of rat calvarial defects. Twenty-four male skeletally-mature Wistar rats were used. Two bone 5 mm critical size defects were created in calvarial bones of each rat. In rats from experimental group I ( = 12), EPO was applied locally on a collagen cone in left defects, whereas a collagen cone soaked with physiological saline was placed in right defects. The rats from experimental group II were injected once intraperitoneally with 4900 IU/kg EPO; a collagen cone was only placed in left defects, whereas the right defects were left empty. The systemic effect of EPO treatment was monitored by haematological analyses on days 0, 30 and 90. Bone healing was monitored via radiography and computed tomography on the same time intervals. The results demonstrated that local EPO application had no significant effect on haemopoiesis, unlike the systemic application. At the same time, it resulted in new bone formation and therefore, could be successfully used as a means of promoting bone regeneration.
本研究旨在比较局部和全身应用重组人促红细胞生成素（rhEPO）对大鼠颅骨缺损愈合的影响。使用了24只雄性骨骼成熟的Wistar大鼠。在每只大鼠的颅骨上制造两个5毫米的临界尺寸骨缺损。在实验组I（n = 12）的大鼠中，将EPO局部应用于左侧缺损处的胶原锥上，而在右侧缺损处放置浸泡有生理盐水的胶原锥。实验组II的大鼠腹腔注射一次4900 IU/kg的EPO；仅在左侧缺损处放置一个胶原锥，而右侧缺损处保持空置。在第0、30和90天通过血液学分析监测EPO治疗的全身效果。在相同的时间间隔通过X射线摄影和计算机断层扫描监测骨愈合情况。结果表明，与全身应用不同，局部应用EPO对造血没有显著影响。同时，它导致了新骨形成，因此，可以成功地用作促进骨再生的手段。
