Vasileva Radina, Chaprazov Tzvetan
Department of Veterinary Surgery, Faculty of Veterinary Medicine, Trakia University, Stara Zagora 6000, Bulgaria.
Vet Sci. 2023 Mar 5;10(3):196. doi: 10.3390/vetsci10030196.
Critical-size bone defect models are the standard in studies of the osteogenic potential of biomaterials. The present investigation aimed to evaluate the ability of recombinant human erythropoietin (EPO) to induce trabecular bone healing either alone or combined with a xenograft in a rat femoral critical-size defect model. Five-mm bone defects were created in the femoral diaphysis of fifty-six skeletally mature male Wistar albino rats. The animals were divided into six groups: one control group and five experimental groups. The defects in the control group were left empty, whereas an absorbable collagen cone soaked either with saline or erythropoietin (alone or in combination with xenograft) was placed in locally treated groups. The systemic treatment group received EPO subcutaneously. Bone formation was objectively evaluated through radiography, osteodensitometry and histological examination on post-operative days 30 and 90. The results demonstrate that EPO, locally applied on a collagen scaffold, was capable of inducing bone healing, whereas the single systemically administered high EPO dose had only an insignificant effect on bone formation. The combination of EPO with a bone substitute under the form of cancellous granules resulted in more rapid integration between the xenograft and host bone.
临界尺寸骨缺损模型是生物材料成骨潜力研究的标准模型。本研究旨在评估重组人促红细胞生成素（EPO）单独或与异种移植物联合应用于大鼠股骨临界尺寸缺损模型中诱导小梁骨愈合的能力。在56只骨骼成熟的雄性Wistar白化大鼠的股骨干中制造5毫米的骨缺损。将动物分为六组：一组对照组和五组实验组。对照组的缺损处不做处理，而在局部治疗组中放置浸泡有生理盐水或促红细胞生成素（单独或与异种移植物联合）的可吸收胶原海绵。全身治疗组皮下注射促红细胞生成素。在术后第30天和第90天通过X线摄影、骨密度测定和组织学检查对骨形成进行客观评估。结果表明，局部应用于胶原支架上的促红细胞生成素能够诱导骨愈合，而单次全身给予高剂量促红细胞生成素对骨形成仅有微不足道的影响。促红细胞生成素与松质颗粒形式的骨替代物联合使用导致异种移植物与宿主骨之间更快地整合。
