Graña-Baumgartner Andrea, Dukkipati Venkata S R, Biggs Patrick J, Kenyon Paul R, Blair Hugh T, López-Villalobos Nicolás, Ross Alastair B
School of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
School of Veterinary Science, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
Animals (Basel). 2022 Oct 14;12(20):2762. doi: 10.3390/ani12202762.
During cold exposure, brown adipose tissue (BAT) holds the key mechanism in the generation of heat, thus inducing thermogenic adaptation in response to cooler environmental changes. This process can lead to a major lipidome remodelling in BAT, where the increase in abundance of many lipid classes plays a significant role in the thermogenic mechanisms for heat production. This study aimed to identify different types of lipids, through liquid chromatography-mass spectrometry (LC-MS), in BAT and plasma during a short-term cold challenge (2-days), or not, in new-born lambs. Fifteen new-born Romney lambs were selected randomly and divided into three groups: Group 1 ( = 3) with BAT and plasma obtained within 24 h after birth, as a control; Group 2 ( = 6) kept indoors for two days at an ambient temperature (20-22 °C) and Group 3 ( = 6) kept indoors for two days at a cold temperature (4 °C). Significant differences in lipid composition of many lipid categories (such as glycerolipids, glycerophospholipids, sphingolipids and sterol lipids) were observed in BAT and plasma under cold conditions, compared with ambient conditions. Data obtained from the present study suggest that short-term cold exposure induces profound changes in BAT and plasma lipidome composition of new-born lambs, which may enhance lipid metabolism via BAT thermogenic activation and adipocyte survival during cold adaptation. Further analysis on the roles of these lipid changes, validation of potential biomarkers for BAT activity, such as LPC 18:1 and PC 35:6, should contribute to the improvement of new-born lamb survival. Collectively, these observations help broaden the knowledge on the variations of lipid composition during cold exposure.
在寒冷暴露期间，棕色脂肪组织（BAT）是产热的关键机制，从而诱导机体对寒冷环境变化产生产热适应。这一过程可导致BAT中主要的脂质组重塑，其中许多脂质种类丰度的增加在产热机制中发挥着重要作用。本研究旨在通过液相色谱-质谱联用（LC-MS）鉴定新生羔羊在短期寒冷刺激（2天）或无寒冷刺激情况下BAT和血浆中的不同类型脂质。随机选择15只新生罗姆尼羔羊，分为三组：第1组（n = 3）在出生后24小时内获取BAT和血浆作为对照；第2组（n = 6）在环境温度（20 - 22°C）下在室内饲养两天；第3组（n = 6）在低温（4°C）下在室内饲养两天。与环境条件相比，在寒冷条件下，BAT和血浆中许多脂质类别（如甘油脂、甘油磷脂、鞘脂和甾醇脂）的脂质组成存在显著差异。本研究获得的数据表明，短期寒冷暴露会引起新生羔羊BAT和血浆脂质组组成的深刻变化，这可能通过BAT产热激活和寒冷适应过程中的脂肪细胞存活来增强脂质代谢。对这些脂质变化作用的进一步分析，以及对BAT活性潜在生物标志物（如LPC 18:1和PC 35:6）的验证，应有助于提高新生羔羊的存活率。总体而言，这些观察结果有助于拓宽对寒冷暴露期间脂质组成变化的认识。
