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原代鼠白色、米色和棕色脂肪细胞的脂类组学——β-肾上腺素能刺激的影响。

The lipidome of primary murine white, brite, and brown adipocytes-Impact of beta-adrenergic stimulation.

机构信息

ZIEL-Institute for Food & Health, Technical University of Munich, Freising, Germany.

Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany.

出版信息

PLoS Biol. 2019 Aug 1;17(8):e3000412. doi: 10.1371/journal.pbio.3000412. eCollection 2019 Aug.

DOI:10.1371/journal.pbio.3000412
PMID:31369546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6692052/
Abstract

Lipid species patterns are conserved within cells to maintain physicochemical properties of membranes and cellular functions. We present the lipidome, including sterols, glycerolipids (GLs), glycerophospholipids (GPLs), and sphingolipids (SLs), of primary ex vivo differentiated (I) white, (II) brite, and (III) brown adipocytes derived from primary preadipocytes isolated from (I) epididymal white, (II) inguinal white, and (III) intrascapular brown adipose tissue. Quantitative lipidomics revealed significantly decreased fractions of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), with longer (C > 36) and more polyunsaturated species, as well as lower levels of cardiolipin (CL) in white than in brite and brown adipocytes. Together, the brite and brown lipidome was comparable and indicates differences in membrane lipid packing density compared with white adipocytes. Changes in ceramide species profile could be related to the degree of browning. Beta-adrenergic stimulation of brown adipocytes led to generation of saturated lyso-PC (LPC) increasing uncoupling protein (UCP) 1-mediated leak respiration. Application of stable isotope labeling showed that LPC formation was balanced by an increased de novo synthesis of PC.

摘要

脂质种类在细胞内保持稳定，以维持膜的物理化学性质和细胞功能。我们展示了源自初级前体脂肪细胞的原代体外分化（I）白色、（II）米色和（III）棕色脂肪细胞的脂质组，包括固醇、甘油酯（GLs）、甘油磷脂（GPLs）和鞘脂（SLs）。定量脂质组学揭示了白色脂肪细胞中磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）的分数明显降低，具有更长（C > 36）和更多多不饱和的物种，以及较低的心磷脂（CL）水平。总的来说，米色和棕色的脂质组相似，表明与白色脂肪细胞相比，膜脂质堆积密度存在差异。神经酰胺种类谱的变化可能与褐色化程度有关。β-肾上腺素能刺激棕色脂肪细胞导致饱和溶血磷脂酰胆碱（LPC）的产生增加解偶联蛋白（UCP）1 介导的泄漏呼吸。稳定同位素标记的应用表明，LPC 的形成通过 PC 的从头合成增加来平衡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/b359e605591b/pbio.3000412.g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/982c90b911b0/pbio.3000412.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/48107f098e97/pbio.3000412.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/a615e903c09f/pbio.3000412.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/b359e605591b/pbio.3000412.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/5b914e1ce2f7/pbio.3000412.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/9d1dc67082c6/pbio.3000412.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/982c90b911b0/pbio.3000412.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/48107f098e97/pbio.3000412.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/a615e903c09f/pbio.3000412.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bfc5/6692052/b359e605591b/pbio.3000412.g006.jpg

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