Ito H, Pitchumoni C S, Glass G B
Am J Dig Dis. 1978 Oct;23(10):919-24. doi: 10.1007/BF01072467.
An autologous "skin window test," used lately for the study of cellular immunity in cancer, was applied here successfully to 54 patients with upper-gastrointestinal ailments, 48 of whom had a coexistent fundic and/or antral chronic gastritis of varying severity. The diagnoses of gastritis were made by multiple fiber-gastroscopic biopsies. The ether-alcohol-fixed cryostat sections of fundic and antral biopsies were mounted on cover slides and placed on small cutaneous abrasions of the forearm of the same patients for 24--28 hr. The exudates on cover slides and on imprints of the abrasions were read blindly for the mononuclear cell response according to criteria set for this test by Black and Leis (10). A positive reaction was obtained in 8 of the 54 patients using autologous fundic mucosal biopsy. An autologous antral mucosal biopsy gave positive reaction in only 2 of the 26 of the patients in whom it was used. The positive yield of this autologous skin window test in chronic advanced fundic gastritis was somewhat higher than that obtained by other authors using lymphocytes blast transformation or macrophages migration inhibition test in vitro. It was much higher than the yield obtained by others who used skin tests in vivo, with homologous or heterologous gastric mucosal extracts as antigens. The autologous skin window is safe in regard to possible transmission of hepatitis. Its applicability for detection of cellular immunity derangement in chronic gastritis carries promise.
最近用于癌症细胞免疫研究的自体“皮肤窗试验”在此成功应用于54例上消化道疾病患者，其中48例同时患有不同严重程度的胃底和/或胃窦慢性胃炎。胃炎的诊断通过多次纤维胃镜活检做出。将胃底和胃窦活检的乙醚-酒精固定低温切片安装在盖玻片上，并放置在同一患者前臂的小皮肤擦伤处24至28小时。根据Black和Leis（10）为此试验设定的标准，对盖玻片和擦伤印记上的渗出物进行单核细胞反应的盲读。在54例使用自体胃底黏膜活检的患者中，8例呈阳性反应。在使用自体胃窦黏膜活检的26例患者中，只有2例呈阳性反应。这种自体皮肤窗试验在慢性进展期胃底胃炎中的阳性率略高于其他作者使用淋巴细胞转化试验或体外巨噬细胞移动抑制试验所获得的阳性率。它远高于其他使用同源或异源胃黏膜提取物作为抗原进行体内皮肤试验的作者所获得的阳性率。自体皮肤窗在肝炎可能传播方面是安全的。其在检测慢性胃炎细胞免疫紊乱方面的适用性具有前景。
