Uibo R M, Krohn K J
Clin Exp Immunol. 1984 Nov;58(2):341-7.
In the present study we used the highly sensitive avidin-biotin complex technique (ABC) for detection of gastrin cell (G cell) autoantibodies. Serum samples were studied from 56 patients with histologically proven antral gastritis, 20 adult subjects with histologically proven normal antral and fundal mucosa and from 25 healthy children. The presence of G cell autoantibodies was assessed by both immunofluorescence (IFL) and the ABC-peroxidase method, using sections from paraformaldehyde-lysine-periodate fixed and paraffin embedded human antral mucosa as antigen. Other organ specific and non-organ specific autoantibodies were demonstrated with IFL. Using the ABC method, G cell autoantibodies were found at a dilution of 1:100 in nine of 56 sera with antral gastritis. One of the control sera was positive. The ABC method was considered superior to IFL in demonstrating G cell antibodies, since positively stained G cells could be easily distinguished from the histiocytic cells in lamina propria which sometimes give false positive staining. Furthermore, higher antibody titres results in the ABC method. Thus it is suggested that by analogy to fundal gastritis, some cases of antral gastritis may have autoimmune aetiology.
在本研究中，我们使用高灵敏度抗生物素蛋白-生物素复合物技术（ABC法）检测胃泌素细胞（G细胞）自身抗体。研究了56例经组织学证实为胃窦炎患者、20例经组织学证实胃窦和胃底黏膜正常的成年受试者以及25名健康儿童的血清样本。以经多聚甲醛-赖氨酸-高碘酸盐固定并石蜡包埋的人胃窦黏膜切片作为抗原，通过免疫荧光法（IFL）和ABC过氧化物酶法评估G细胞自身抗体的存在。用IFL检测其他器官特异性和非器官特异性自身抗体。采用ABC法，在56例胃窦炎患者的血清中，有9例在1:100稀释度时检测到G细胞自身抗体。1份对照血清呈阳性。在检测G细胞抗体方面，ABC法被认为优于IFL法，因为阳性染色的G细胞很容易与固有层中的组织细胞区分开来，后者有时会产生假阳性染色。此外，ABC法检测出的抗体滴度更高。因此，有人提出，与胃底炎类似，某些胃窦炎病例可能有自身免疫病因。
