Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
ACS Appl Mater Interfaces. 2022 Jan 12;14(1):337-349. doi: 10.1021/acsami.1c20719. Epub 2021 Dec 30.
Weak interactions play an important role in soft corona (SC) formation and thus help in evaluating the biological fate of the nanoparticles (NPs). Preadsorption of specific proteins on the NP surface, leading to SC formation, has been found to help NPs in evading immunosurveillance. However, the role of different preadsorbed biomolecules in determining the NP pathophysiology and cellular association, upon their re-exposure to conditions, still remains elusive. Here, differently charged gold NPs were precoated with two different blood components, red blood cells and human serum albumin protein, and these were then re-exposed to human serum. Cloaking NPs with protein improved the NP colloidal stability and other physico-chemical properties along with increased cellular association. Detailed proteomic analysis suggested that protein-camouflaged NPs showed a decrease in immune-responsive proteins compared to their bare counterparts. Further, it was also observed that the secondary protein signature on the NP surface was governed by primary protein coating; however, the event was more or less NP charge-independent. This study will pave the path for future strategies to make NPs invincible to the immunosurveillance system of the body.
弱相互作用在软 corona (SC) 的形成中起着重要作用，因此有助于评估纳米粒子 (NPs) 的生物学命运。在 NP 表面预先吸附特定的蛋白质，导致 SC 的形成，已被发现有助于 NPs 逃避免疫监视。然而，不同预先吸附的生物分子在决定 NP 的病理生理学和细胞关联方面的作用，在它们重新暴露于条件下，仍然难以捉摸。在这里，不同带电的金纳米粒子预先用两种不同的血液成分，红细胞和人血清白蛋白蛋白进行了涂覆，然后将其重新暴露于人血清中。用蛋白质包裹纳米粒子提高了 NP 的胶体稳定性和其他物理化学性质，同时增加了细胞的关联。详细的蛋白质组学分析表明，与裸纳米粒子相比，蛋白质伪装纳米粒子的免疫反应性蛋白减少。此外，还观察到 NP 表面的二级蛋白质特征受一级蛋白质涂层的控制；然而，这种情况或多或少与 NP 电荷无关。这项研究将为未来的策略铺平道路，使 NPs 不受身体免疫监视系统的影响。
