Xu Xuan, Song Huayu, Zhang Lu, Chen Chonghui, Zhang Xiaoxu, Liu Yiying, Li Chao, Fu Qiang
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Qingdao Conson Oceantec Valley Development Co., Ltd., Qingdao 266237, China.
Genes (Basel). 2025 Mar 29;16(4):392. doi: 10.3390/genes16040392.
As an innovative approach to deep-sea aquaculture, fish farm vessels offer a dual benefit by alleviating the pressure on offshore fishing resources while providing an additional high-quality protein source. However, the potential impacts of vessel coatings on farmed fish remain poorly understood. In this study, to investigate the effects of vessel coatings on the large yellow croaker (), we established four experimental groups with coating concentrations at 1-fold, 10-fold, 20-fold, and 80-fold levels. Antioxidant enzyme activities in kidney tissues were measured across all groups, while histological and transcriptome analyses were specifically conducted for the 1-fold and 80-fold concentration groups. Firstly, significant alterations in antioxidant enzyme activity were observed in the 80-fold concentration group. Moreover, histological analysis demonstrated more severe pathological changes in kidney tissue at the higher concentration, including interstitial hemorrhage and tubular epithelial cell fatty degeneration. In addition, we identified 11,902 differentially expressed genes (DEGs) by high-throughput sequencing. KEGG pathway enrichment analysis revealed that the DEGs were predominantly involved in critical biological processes, including endoplasmic reticulum protein processing, oxidative phosphorylation, cytokine-cytokine receptor interactions, cell cycle regulation, DNA replication, and PPAR signaling pathways. Finally, the validation of nine selected DEGs through quantitative real-time PCR (qRT-PCR) showed significant correlation with RNA-Seq data, confirming the reliability of our transcriptome analysis. This study provides preliminary insights into the antioxidant stress response mechanisms of to coating exposure and establishes a theoretical foundation for optimizing healthy fish farming practices in aquaculture vessels.
作为深海养殖的一种创新方法，养鱼船具有双重益处，既能减轻近海渔业资源压力，又能提供额外的优质蛋白质来源。然而，船体涂层对养殖鱼类的潜在影响仍知之甚少。在本研究中，为了探究船体涂层对大黄鱼的影响，我们设立了四个实验组，涂层浓度分别为1倍、10倍、20倍和80倍水平。对所有组的肾脏组织中的抗氧化酶活性进行了测定，同时对1倍和80倍浓度组专门进行了组织学和转录组分析。首先，在80倍浓度组中观察到抗氧化酶活性有显著变化。此外，组织学分析表明，较高浓度下肾脏组织的病理变化更为严重，包括间质出血和肾小管上皮细胞脂肪变性。另外，通过高通量测序我们鉴定出11902个差异表达基因（DEGs）。KEGG通路富集分析显示，这些差异表达基因主要参与关键生物学过程，包括内质网蛋白加工、氧化磷酸化、细胞因子-细胞因子受体相互作用、细胞周期调控、DNA复制和PPAR信号通路。最后，通过定量实时PCR（qRT-PCR）对九个选定的差异表达基因进行验证，结果显示与RNA测序数据有显著相关性，证实了我们转录组分析的可靠性。本研究为大黄鱼对涂层暴露的抗氧化应激反应机制提供了初步见解，并为优化水产养殖船中健康养鱼实践奠定了理论基础。
