Jiangsu Lixiahe District Institute of Agricultural Sciences, Yangzhou, 225007, China.
Mol Immunol. 2021 Jan;129:63-69. doi: 10.1016/j.molimm.2020.10.020. Epub 2020 Nov 20.
Cnaphalocrocis medinalis granulovirus (CnmeGV) is a potential microbial agent against the rice leaffolder. Innate immunity is essential for insects to survive pathogenic infection. Therefore, to clarify the immune response of Cnaphalocrocis medinalis to the viral colonization, the gene expression profile of C. medinalis infected with CnmeGV was constructed by RNA-seq. A total of 8,503 differentially expressed genes (DEGs) were found including 5,304 up-regulated and 3,199 down-regulated unigenes. Gene enrichment analysis indicated that these DEGs were mainly linked to protein synthesis and metabolic process as well as ribosome and virus-infection pathways. Specifically, a significantly up-regulated PiggyBac-like transposon gene was identified suggested that the enhancement of transposon activity is related to host immunity. Further, the DEGs encoding oxidative stress related genes were identified and validated by RT-qPCR. Overall, 9 antioxidant enzyme genes and 4 antioxidant protein genes were up-regulated, and the extensive glutathione S-transferase genes were down-regulated. Our results provide a basis for understanding the molecular mechanisms of baculovirus action and oxidative stress response in C. medinalis and other insects.
中肠绒茧蜂颗粒体病毒（CnmeGV）是一种针对水稻螟虫的潜在微生物制剂。先天免疫对于昆虫抵御病原感染至关重要。因此，为了阐明中肠绒茧蜂对病毒定植的免疫反应，本研究通过 RNA-seq 构建了感染 CnmeGV 的中肠绒茧蜂的基因表达谱。共发现了 8503 个差异表达基因（DEGs），包括 5304 个上调基因和 3199 个下调基因。基因富集分析表明，这些 DEGs 主要与蛋白质合成和代谢过程以及核糖体和病毒感染途径有关。具体来说，鉴定到一个显著上调的 PiggyBac 样转座子基因，表明转座子活性的增强与宿主免疫有关。此外，通过 RT-qPCR 验证和鉴定了编码氧化应激相关基因的 DEGs。总体而言，9 个抗氧化酶基因和 4 个抗氧化蛋白基因上调，广泛的谷胱甘肽 S-转移酶基因下调。本研究结果为理解杆状病毒在中肠绒茧蜂和其他昆虫中的作用机制以及氧化应激反应提供了基础。
