Sengupta Madhumita, Vimal Neha, Angmo Nilza, Seth Rakesh Kumar
Applied Entomology and Radiation Biology Lab, Department of Zoology, University of Delhi, Delhi 110007, India.
Insects. 2022 Oct 2;13(10):898. doi: 10.3390/insects13100898.
Radiobiological investigations on the reproductive behavior of female (Fabr.) were conducted with the aim of determining the suitable radio-sterilizing dose for females in order to release them along with sub-sterile males for effective implementation of the Inherited Sterility technique against this pest. Calling and copulation duration significantly increased, while mating success, oviposition, fertility and longevity significantly decreased with increasing radiation dose (100-200 Gy) compared to control. In view of the effect of irradiation on mating behavior and reproductive viability of female , 130 Gy was identified as a suitable radio-sterilization dose. Further molecular studies were conducted to corroborate this dose for female sterilization, along with a higher dose of 200 Gy in order to validate the gradational response of ionizing radiation. GC-MS analysis indicated decreased sex pheromone titer at 130 Gy, which was more pronounced at 200 Gy. Pheromone-associated genes, and showed decreased expression at 130 Gy, and were drastically reduced at 200 Gy. The fertility-related gene also showed a negative correlation with radiation exposure. Based on these radiation responses of female , 130 Gy might be considered a suitable dose for complete female sterility and its inclusion in sterile insect programs against .
对雌性（法布尔）的生殖行为进行了放射生物学研究，目的是确定适合雌性的辐射绝育剂量，以便与亚不育雄性一起释放，从而有效实施针对这种害虫的遗传不育技术。与对照相比，随着辐射剂量（100 - 200 Gy）增加，求偶和交配持续时间显著增加，而交配成功率、产卵量、繁殖力和寿命显著下降。鉴于辐射对雌性交配行为和生殖活力的影响，130 Gy被确定为合适的辐射绝育剂量。进一步进行了分子研究，以证实该剂量对雌性绝育的效果，同时研究了更高剂量200 Gy，以验证电离辐射的分级反应。气相色谱 - 质谱分析表明，130 Gy时性信息素滴度降低，在200 Gy时更为明显。与信息素相关的基因在130 Gy时表达下降，在200 Gy时大幅降低。与繁殖力相关的基因也与辐射暴露呈负相关。基于雌性的这些辐射反应，130 Gy可能被认为是使雌性完全不育并将其纳入针对该害虫的不育昆虫计划的合适剂量。
