Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Nasr City, Egypt.
Int J Radiat Biol. 2021;97(3):376-385. doi: 10.1080/09553002.2021.1864046. Epub 2021 Jan 6.
This study aimed to investigate the impact of gamma radiation of males (resulted from irradiated pupae) crossed with unirradiated females on fecundity, egg hatchability, adult emergence, sex ratio, sterility, in addition to reproductive development at the level of oogenesis and spermatogenesis compared to unirradiated group.
The housefly, pupae were exposed to three sublethal doses of 5, 10, and 15 Gy.
Fecundity was severely reduced particularly in F2 (11.33 ± 1.528; 7.33 ± 1.115 eggs/♀) and F3 (9.0 ± 1.00; 4.67 ± 1.115 eggs/♀) for doses of 10 and 15 Gy, respectively, compared with (52.0 ± 1.4 eggs/♀) for the control. Data revealed latent dose- and generation-dependent reduction in egg hatchability. Hatchability percentages reduced from 93.59 for the control to 10.07 (F1), 8.09 (F2), and 8.34 (F3) when the highest radiation dose 15 Gy was applied. Irradiation induced paternal deleterious substerility effects. Irradiation with 15 Gy induced substerility that reached about 97.0% in F2 and F3 generations. A significant ( < 0.05) reduction of the mean numbers of adult emergence was remarkably detected in the F1, F2, and F3 generations. Applied gamma doses did not affect the male to female ratio in the Parental or F1 generations. However, the F2 and F3 generations did show changes to the sex ratio with males occurring more frequently than females. This trend became more pronounced as dose increased. Ultrastructural examinations exhibited unusual damage and malformation either for males or female reproductive organs.
The obtained results clearly show that gamma radiation of irradiated as pupae induced considerably visible impact on tested biological aspects and reproductive potential.
本研究旨在探讨雄性（由辐照蛹产生）与未辐照雌性交配对生育力、卵孵化率、成虫出现率、性别比、不育率以及卵发生和精子发生生殖发育的影响，与未辐照组相比。
将家蝇蛹暴露于三个亚致死剂量的 5、10 和 15Gy。
生育力严重降低，特别是在 F2（11.33±1.528；7.33±1.115 个/♀）和 F3（9.0±1.00；4.67±1.115 个/♀）中，与对照组（52.0±1.4 个/♀）相比，10 和 15Gy 的剂量分别。数据显示，卵孵化率随潜伏剂量和世代的减少而降低。孵化率百分比从对照组的 93.59%降低到 10.07%（F1）、8.09%（F2）和 8.34%（F3），当应用最高辐射剂量 15Gy 时。照射诱导了父系有害的亚不育效应。用 15Gy 照射诱导的亚不育在 F2 和 F3 代中达到约 97.0%。在 F1、F2 和 F3 代中，成虫出现的平均数量明显减少，差异显著（<0.05）。应用的伽马剂量对亲本或 F1 代的雌雄比例没有影响。然而，F2 和 F3 代的性别比例发生了变化，雄性比雌性更常见。这种趋势随着剂量的增加而变得更加明显。超微结构检查显示，雄性或雌性生殖器官都出现了异常的损伤和畸形。
研究结果表明，辐照蛹的γ辐射对测试的生物学方面和生殖潜力产生了相当明显的影响。
