Instituto Cajal-CSIC, Madrid, Spain.
Instituto Cajal-CSIC, Madrid, Spain.
Stem Cell Reports. 2019 Oct 8;13(4):700-712. doi: 10.1016/j.stemcr.2019.08.010. Epub 2019 Sep 19.
Understanding the contribution of adult neural progenitor cells (NPCs) and their lineage potential is a great challenge in neuroscience. To reveal progenitor diversity and cell-lineage relationships of postnatal NPCs in the subventricular zone (SVZ), we performed in vivo lineage-tracing genetic analysis using the UbC-StarTrack. We determined the progeny of single SVZ-NPCs, the number of cells per clone, the dispersion of sibling cells, and the cell types within clones. Long-term analysis revealed that both the cell-dispersion pattern and number of cells comprising clones varied depending on the glial/neuronal nature of sibling cells. Sibling-olfactory interneurons were primarily located within the same layer, while sibling-glial cells populated SVZ-adjacent areas. Sibling astrocytes and interneurons did not form big clones, whereas oligodendroglial-lineage clones comprised the largest clones originated in adult brains. These results demonstrate the existence of SVZ postnatal bipotential progenitors that give rise to clones widely dispersed across the olfactory bulb and SVZ-adjacent areas.
理解成年神经祖细胞 (NPCs) 的贡献及其谱系潜能是神经科学面临的一大挑战。为了揭示室下区 (SVZ) 中 NPC 后代的祖细胞多样性和细胞谱系关系，我们使用 UbC-StarTrack 进行了体内谱系追踪遗传分析。我们确定了单个 SVZ-NPC 的后代、每个克隆的细胞数量、同胞细胞的分散程度以及克隆内的细胞类型。长期分析表明，克隆中细胞分散模式和细胞数量的变化取决于同胞细胞的胶质/神经元性质。同胞嗅觉中间神经元主要位于同一层，而同胞神经胶质细胞则分布在 SVZ 附近区域。同胞星形胶质细胞和中间神经元不会形成大的克隆，而少突胶质细胞谱系克隆则包含起源于成年大脑的最大克隆。这些结果表明，SVZ 存在具有多潜能的祖细胞，它们可以产生广泛分布于嗅球和 SVZ 附近区域的克隆。
