School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China.
School of Exercise and Health, Guangzhou Sport University, Guangzhou 510500, China.
Int J Mol Sci. 2022 Oct 20;23(20):12608. doi: 10.3390/ijms232012608.
Cardiovascular diseases are associated with platelet hyperactivity, and downregulating platelet activation is one of the promising antithrombotic strategies. This study newly extracted two polysaccharides (purified exopolysaccharides, EPSp and purified intercellular exopolysaccharides, IPSp) from Cs-4 mycelial fermentation powder, and investigated the effects of the two polysaccharides and their gut bacterial metabolites on platelet functions and thrombus formation. EPSp and IPSp are majorly composed of galactose, mannose, glucose, and arabinose. Both EPSp and IPSp mainly contain 4-Gal and 4-Glc glycosidic linkages. EPSp and IPSp significantly inhibited human platelet activation and aggregation with a dose-dependent manner, and attenuated thrombus formation in mice without increasing bleeding risk. Furthermore, the EPSp and IPSp after fecal fermentation showed enhanced platelet inhibitory effects. The results have demonstrated the potential value of Cs-4 polysaccharides as novel protective ingredients for cardiovascular diseases.
心血管疾病与血小板活性过高有关，而抑制血小板活化是一种有前途的抗血栓策略。本研究从 Cs-4 菌丝发酵粉中新提取了两种多糖（纯化胞外多糖，EPSp 和纯化胞间胞外多糖，IPSp），并研究了这两种多糖及其肠道细菌代谢物对血小板功能和血栓形成的影响。EPSp 和 IPSp 主要由半乳糖、甘露糖、葡萄糖和阿拉伯糖组成。EPSp 和 IPSp 主要含有 4-Gal 和 4-Glc 糖苷键。EPSp 和 IPSp 呈剂量依赖性显著抑制人血小板活化和聚集，并减轻小鼠血栓形成而不增加出血风险。此外，粪便发酵后的 EPSp 和 IPSp 显示出增强的血小板抑制作用。这些结果表明 Cs-4 多糖作为心血管疾病新型保护成分具有潜在价值。
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