Mao Yu-Heng, Xu Yixuan, Song Fenglin, Wang Zhao-Mei, Li Yan-Heng, Zhao Mingzhu, He Fang, Tian Zezhong, Yang Yan
School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University (Northern Campus), Guangzhou, Guangdong Province 510080, China; Guangdong Engineering Technology Research Center of Nutrition Translation, Guangzhou, Guangdong Province 510080, China.
School of Food Science, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China.
Carbohydr Polym. 2022 Aug 15;290:119476. doi: 10.1016/j.carbpol.2022.119476. Epub 2022 Apr 12.
This study assessed the protective effects of konjac glucomannan (KGM) on gut microbiome against the antibiotic perturbation in C57BL/6J mice. The native KGM (1.82 × 10) was partially hydrolyzed by endo-1,4-β-mannanase, and two hydrolyzed fractions (KGM-eM with 3.82 × 10 Da and KGM-eL with 8.27 × 10 Da) were characterized and applied to mice with perturbation of antibiotics in comparison with the native KGM. The results showed that the native KGM better maintained the microbial diversity and composition in feces, and increased the production of the individual and total SCFAs in feces and serum with perturbation of antibiotics. In contrast, KGM with lower MW (KGM-eM and KGM-eL) increased the proportion of Lactobacillus and SCFA production with no antibiotics, however, the prebiotic effects were eliminated with perturbation of antibiotics. These results have demonstrated the protective effects of KGM with high MW on gut microbiome against the antibiotic perturbation in vivo.
本研究评估了魔芋葡甘聚糖（KGM）对C57BL/6J小鼠肠道微生物群免受抗生素干扰的保护作用。天然KGM（1.82×10）被内切-1,4-β-甘露聚糖酶部分水解，对两个水解组分（分子量为3.82×10 Da的KGM-eM和分子量为8.27×10 Da的KGM-eL）进行了表征，并与天然KGM相比应用于受抗生素干扰的小鼠。结果表明，天然KGM能更好地维持粪便中的微生物多样性和组成，并在抗生素干扰的情况下增加粪便和血清中单个和总短链脂肪酸的产生。相比之下，较低分子量的KGM（KGM-eM和KGM-eL）在无抗生素的情况下增加了乳酸杆菌的比例和短链脂肪酸的产生，然而，在抗生素干扰的情况下益生元效应消失。这些结果证明了高分子量KGM对体内肠道微生物群免受抗生素干扰的保护作用。
