Artificial intelligence (AI)-assisted diagnosis and treatment could expand the medical scenarios and augment work efficiency and accuracy. However, factors influencing healthcare workers' adoption intention of AI-assisted diagnosis and treatment are not well-understood. This study conducted a cross-sectional study of 343 dental healthcare workers from tertiary hospitals and secondary hospitals in Anhui Province. The obtained data were analyzed using structural equation modeling. The results showed that performance expectancy and effort expectancy were both positively related to healthcare workers' adoption intention of AI-assisted diagnosis and treatment. Social influence and human-computer trust, respectively, mediated the relationship between expectancy (performance expectancy and effort expectancy) and healthcare workers' adoption intention of AI-assisted diagnosis and treatment. Furthermore, social influence and human-computer trust played a chain mediation role between expectancy and healthcare workers' adoption intention of AI-assisted diagnosis and treatment. Our study provided novel insights into the path mechanism of healthcare workers' adoption intention of AI-assisted diagnosis and treatment.