Li Qian, Chen Xi, Lin Lianyu, Zhang Lianhu, Wang Li, Bao Jiandong, Zhang Dongmei
Meishan Vocational Technical College, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Fungi (Basel). 2022 Oct 11;8(10):1060. doi: 10.3390/jof8101060.
The small Rho GTPase acts as a molecular switch in eukaryotic signal transduction, which plays a critical role in polar cell growth and vesicle trafficking. Previous studies demonstrated that constitutively active (CA) mutant strains, of were defective in appressorium formation. While dominant-negative (DN) mutant strains shows defects in polar growth. However, the molecular dynamics of MoRho3-mediated regulatory networks in the pathogenesis of still needs to be uncovered. Here, we perform comparative transcriptomic profiling of and mutant strains using a high-throughput RNA sequencing approach. We find that genetic manipulation of MoRho3 significantly disrupts the expression of 28 homologs of Rho3-interacting proteins, including EXO70, BNI1, and BNI2 in the , mutant strains. Functional enrichment analyses of up-regulated DEGs reveal a significant enrichment of genes associated with ribosome biogenesis in the mutant strain. Down-regulated DEGs in the mutant strains shows significant enrichment in starch/sucrose metabolism and the ABC transporter pathway. Moreover, analyses of down-regulated DEGs in the in reveals an over-representation of genes enriched in metabolic pathways. In addition, we observe a significant suppression in the expression levels of secreted proteins suppressed in both and mutant strains. Together, our results uncover expression dynamics mediated by two states of the small GTPase MoRho3, demonstrating its crucial roles in regulating the expression of ribosome biogenesis and secreted proteins.
小Rho GTPase在真核信号转导中充当分子开关，在极性细胞生长和囊泡运输中起关键作用。先前的研究表明，组成型激活（CA）突变菌株在附着胞形成方面存在缺陷。而显性负性（DN）突变菌株在极性生长方面表现出缺陷。然而，MoRho3介导的调控网络在发病机制中的分子动力学仍有待揭示。在此，我们使用高通量RNA测序方法对MoRho3突变菌株和野生型菌株进行了比较转录组分析。我们发现，对MoRho3进行基因操作会显著破坏MoRho3相互作用蛋白的28个同源物的表达，包括MoRho3突变菌株和野生型菌株中的EXO70、BNI1和BNI2。对上调的差异表达基因进行功能富集分析发现，MoRho3突变菌株中与核糖体生物发生相关的基因显著富集。MoRho3突变菌株中下调的差异表达基因在淀粉/蔗糖代谢和ABC转运蛋白途径中显著富集。此外，对野生型菌株中下调的差异表达基因的分析揭示了富集在代谢途径中的基因的过度表达。此外，我们观察到MoRho3突变菌株和野生型菌株中分泌蛋白的表达水平均受到显著抑制。总之，我们的结果揭示了小GTPase MoRho3两种状态介导的表达动态，证明了其在调节核糖体生物发生和分泌蛋白表达中的关键作用。
