Huang Qiang, Zhu Yong-Qiang, Fouks Bertrand, He Xu-Jiang, Niu Qing-Sheng, Zheng Hua-Jun, Zeng Zhi-Jiang
Honeybee Research Institute, Jiangxi Agricultural University, Zhimin Ave. 1101, Nanchang 330045, China.
Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Zhimin Ave. 1101, Nanchang 330045, China.
Life (Basel). 2022 Oct 19;12(10):1642. doi: 10.3390/life12101642.
The technology of long reads substantially improved the contingency of the genome assembly, particularly resolving contiguity of the repetitive regions. By integrating the interactive fragment using Hi-C, and the HiFi technique, a solid genome of the honeybee was assembled at the chromosomal level. A distinctive pattern of genes involved in social evolution was found by comparing it with social and solitary bees. A positive selection was identified in genes involved with cold tolerance, which likely underlies the adaptation of this European honeybee subspecies in the north hemisphere. The availability of this new high-quality genome will foster further studies and advances on genome variation during subspeciation, honeybee breeding and comparative genomics.
长读长技术极大地改善了基因组组装的连续性，特别是解决了重复区域的连续性问题。通过利用Hi-C技术整合交互式片段以及HiFi技术，在染色体水平上组装了一个高质量的蜜蜂基因组。通过将其与群居和独居蜜蜂进行比较，发现了参与社会进化的基因的独特模式。在与耐寒性相关的基因中鉴定出正选择，这可能是这种欧洲蜜蜂亚种在北半球适应的基础。这个新的高质量基因组的可用性将促进亚种形成、蜜蜂育种和比较基因组学过程中基因组变异的进一步研究和进展。
