NFκB1基因多态性与加拿大人群中甲型流感病毒（H1N1）的严重感染相关。

NFκB1 Polymorphisms Are Associated with Severe Influenza A (H1N1) Virus Infection in a Canadian Population.

作者信息

Mirzo Suhrobjon Mullo, Kumar Anand, Sharma Naresh Kumar, Li Lin, Balshaw Robert, Plummer Francis A, Luo Ma, Liang Binhua

机构信息

Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3X 0P3, Canada.

Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.

出版信息

Microorganisms. 2022 Sep 21;10(10):1886. doi: 10.3390/microorganisms10101886.

DOI:10.3390/microorganisms10101886

PMID:36296162

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9606957/

Abstract

Background: We examined associations between NFκB1 polymorphisms and influenza A (H1N1) clinical outcomes in Canadian. Methods: A total of thirty-six Caucasian patients admitted to the intensive care unit (ICU) in hospitals in Canada were recruited during the 2009 H1N1 pandemic. Genomic DNA was extracted from the whole blood samples. The NFkB1 gene was targeted for genotyping using next-generation sequencing technology—Roche 454. Results: A total of 136 single nucleotide polymorphisms (SNPs) were discovered within the NFκB1 gene. Among them, 63 SNPs were significantly enriched in patients admitted in the ICU (p < 0.05) compared with the British Caucasian population in the 1000 Genomes study. These enriched SNPs are mainly intron variants, and only two are exon SNPs from the non-transcribing portion of the NFκB1 gene. Conclusions: Genetic variations in the NFκB1 gene could influence clinical outcomes of pandemic H1N1 infections. Our findings showed that sequence variations of the NFκB1 gene might influence patient response to influenza infection.

摘要

背景

我们研究了加拿大人群中NFκB1基因多态性与甲型H1N1流感临床结局之间的关联。方法：在2009年H1N1流感大流行期间，招募了加拿大医院重症监护病房（ICU）收治的36名白种人患者。从全血样本中提取基因组DNA。使用下一代测序技术——罗氏454对NFkB1基因进行基因分型。结果：在NFκB1基因内共发现136个单核苷酸多态性（SNP）。其中，与千人基因组研究中的英国白种人群相比，63个SNP在ICU收治的患者中显著富集（p < 0.05）。这些富集的SNP主要是内含子变异，只有两个是来自NFκB1基因非转录部分的外显子SNP。结论：NFκB1基因的遗传变异可能影响甲型H1N1流感大流行感染的临床结局。我们的研究结果表明，NFκB1基因的序列变异可能影响患者对流感感染的反应。