Zhang Mingzhu, Peng Chao, Sun Wentao, Dong Rui, Hao Jun
College of Animal Science, Guizhou University, Guiyang 550025, China.
Microorganisms. 2022 Oct 13;10(10):2023. doi: 10.3390/microorganisms10102023.
Plant phyllosphere bacteria are vital for plant health and productivity and are affected by both abiotic and biotic factors. In this study, we surveyed the structure of the phyllosphere bacterial community associated with alfalfa. For two varieties of alfalfa, forty-eight samples of phyllosphere communities were collected at two locations over four seasons in 2020. Proteobacteria and actinobacteria were associated with the dominating phylum in the bacterial communities of the alfalfa phyllosphere. was the most abundant genus-level bacteria, followed by , , and . Sampling time had a greater affect than site and variety on alfalfa surface microorganisms. The variation in phyllosphere bacterial community assembly was mostly explained by the season-site interaction (43%), season-variety interaction (35%), and season (28%). Variety, site-variety interaction, and season-site-variety interactions did not have a meaningful effect on phyllosphere bacterial diversity and community structure. The bacterial community in the phyllosphere of alfalfa showed seasonal changes over time. The environmental factors that contributed most to the phyllosphere bacterial community of alfalfa were temperature and sunshine duration, which were significantly positively correlated with most of the dominant bacterial genera in the alfalfa phyllosphere.
植物叶际细菌对植物健康和生产力至关重要，并受到非生物和生物因素的影响。在本研究中，我们调查了与苜蓿相关的叶际细菌群落结构。对于两个苜蓿品种，2020年在两个地点的四个季节共采集了48个叶际群落样本。变形菌门和放线菌门是苜蓿叶际细菌群落中的优势菌门。 是属水平上最丰富的细菌，其次是 、 和 。采样时间对苜蓿表面微生物的影响大于地点和品种。叶际细菌群落组装的变化主要由季节-地点相互作用（43%）、季节-品种相互作用（35%）和季节（28%）解释。品种、地点-品种相互作用和季节-地点-品种相互作用对叶际细菌多样性和群落结构没有显著影响。苜蓿叶际的细菌群落随时间呈现季节性变化。对苜蓿叶际细菌群落贡献最大的环境因素是温度和日照时长，它们与苜蓿叶际的大多数优势细菌属显著正相关。
