Zhou Yixin, Shi Zhichao, Pang Qiliang, Liang Xiufeng, Li Hongtao, Sui Xin, Li Chongwei, Song Fuqiang
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China.
Heilongjiang Province Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Microorganisms. 2022 Oct 18;10(10):2059. doi: 10.3390/microorganisms10102059.
Mycorrhiza helper bacteria (MHB) play an important role in driving mycorrhizal formation. There are few reports on the relationship between bacteria and fruiting growths. Taking mycorrhizal rhizosphere soil from sporocarps of the S. luteus and non-mycorrhizal rhizosphere soil of the host plant (Larix gmelinii), we measured the bacterial community structure and diversity and chemical properties to clarify the effect of bacteria on fruiting-body formation. The bacterial diversity was significantly higher in mycorrhizal rhizosphere soil (p < 0.05) than that in non-mycorrhizal rhizosphere soil. The relative abundance of Burkholderia, Bradyrhizobium, Pseudomonas, and Rhizobium was significantly higher (p < 0.05) in mycorrhizal rhizosphere soil than in non-mycorrhizal rhizosphere soil. The soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), ammonium nitrogen (AN), available phosphorus (AP), available potassium (AK), and the activity of catalase, urease, and phosphatase in mycorrhizal rhizosphere soil were significantly higher (p < 0.05) than those in non-mycorrhizal rhizosphere soil. A redundancy analysis (RDA) showed that dominant bacteria are closely related to soil enzyme activity and physicochemical properties (p < 0.05). The boletus recruits a large number of bacteria around the plant roots that speed up nutrient transformation and increase the soil nutrient content, providing an important guarantee for mycelium culture and fruiting-body formation. These findings provide ideas for the nutritional supply of boletus sporocarps and lay the theoretical foundation for the efficient artificial cultivation of boletus.
菌根辅助细菌（MHB）在促进菌根形成方面发挥着重要作用。关于细菌与子实体生长之间关系的报道较少。我们采集了黄粘盖牛肝菌子实体的菌根根际土壤以及寄主植物（落叶松）的非菌根根际土壤，测量了细菌群落结构、多样性和化学性质，以阐明细菌对子实体形成的影响。菌根根际土壤中的细菌多样性显著高于非菌根根际土壤（p < 0.05）。伯克霍尔德氏菌属、慢生根瘤菌属、假单胞菌属和根瘤菌属在菌根根际土壤中的相对丰度显著高于非菌根根际土壤（p < 0.05）。菌根根际土壤中的土壤有机质（SOM）、全氮（TN）、全磷（TP）、全钾（TK）、铵态氮（AN）、有效磷（AP）、有效钾（AK）以及过氧化氢酶、脲酶和磷酸酶的活性均显著高于非菌根根际土壤（p < 0.05）。冗余分析（RDA）表明，优势细菌与土壤酶活性和理化性质密切相关（p < 0.05）。牛肝菌在植物根系周围募集了大量细菌，加速了养分转化并增加了土壤养分含量，为菌丝体培养和子实体形成提供了重要保障。这些研究结果为牛肝菌子实体的营养供应提供了思路，为牛肝菌的高效人工栽培奠定了理论基础。
