研究大黄及其衍生成分大黄素在高脂饮食和链脲佐菌素诱导的糖尿病大鼠中的化学成分、降血糖和降血脂作用。
Investigation of the Chemical Composition, Antihyperglycemic and Antilipidemic Effects of and Its Derived Constituent, Umbelliferone on High-Fat Diet and Streptozotocin-Induced Diabetic Rats.
机构信息
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
出版信息
Molecules. 2022 Oct 16;27(20):6941. doi: 10.3390/molecules27206941.
This study was designed to investigate the chemical profile, antihyperglycemic and antilipidemic effect of total methanolic extract (TME) of and isolated pure compound umbelliferone (UFN) in high-fat diet (HFD)- and streptozotocin (STZ)- induced diabetic rats. TME was subjected to various techniques of chromatography to yield UFN. Diabetes was induced after eight weeks of HFD by administration of STZ (40 mg/kg) intraperitoneally, and experimental subjects were divided into five groups. The diabetic control showed an increase in levels of blood glucose throughout the experiment. Treatments were initiated in the other four groups with glibenclamide (GLB) (6 mg/kg), TME (200 mg/kg and 400 mg/kg) and isolated UFN (50 mg/kg) orally. The effect on blood glucose, lipid profile and histology of the pancreatic and adipose tissues was assessed. Both 200 and 400 mg/kg of TME produced a comparably significant decrease in blood glucose levels and an increase in insulin levels with GLB. UFN began to show a better blood sugar-lowering effect after 14 days of treatment, comparatively. However, both 400 mg/kg TME and UFN significantly returned blood glucose levels in diabetic rats compared to normal rats. Analysis of the lipid profile showed that while HFD + STZ increased all lipid profile parameters, TME administration produced a significant decrease in their levels. Histopathological examinations showed that treatment with TME and UFN revealed an improved cellular architecture, with the healthy islets of Langerhans and compact glandular cells for pancreatic cells distinct from damaged cells in non-treated groups. Conversely, the adipose tissue displayed apparently normal polygonal fat cells. Therefore, these results suggest that TME has the potential to ameliorate hyperglycemia conditions and control lipid profiles in HFD + STZ-induced diabetic rats.
本研究旨在探讨 全甲醇提取物（TME）和分离得到的纯化合物 Umbelliferone（UFN）在高脂肪饮食（HFD）和链脲佐菌素（STZ）诱导的糖尿病大鼠中的化学特征、降血糖和降血脂作用。TME 经各种色谱技术处理得到 UFN。八周后，通过腹腔注射 STZ（40mg/kg）诱导 HFD 诱导糖尿病，实验对象分为五组。糖尿病对照组在整个实验过程中血糖水平升高。另外四组开始给予格列本脲（GLB）（6mg/kg）、TME（200mg/kg 和 400mg/kg）和分离得到的 UFN（50mg/kg）口服治疗。评估血糖、血脂谱以及胰腺和脂肪组织的组织学变化。TME 200mg/kg 和 400mg/kg 与 GLB 相比，可显著降低血糖水平，增加胰岛素水平。与正常大鼠相比，UFN 在治疗 14 天后才开始显示出更好的降血糖作用。然而，TME 400mg/kg 和 UFN 均能显著降低糖尿病大鼠的血糖水平。血脂谱分析表明，HFD+STZ 增加了所有血脂谱参数，而 TME 给药则显著降低了它们的水平。组织病理学检查显示，TME 和 UFN 治疗可改善胰岛细胞的结构，健康的胰岛和致密的腺细胞明显不同于未治疗组受损的细胞。相反，脂肪组织显示出明显正常的多边形脂肪细胞。因此，这些结果表明 TME 具有改善 HFD+STZ 诱导的糖尿病大鼠高血糖状态和控制血脂谱的潜力。
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