Alafnan Ahmed, Rizvi Syed Mohd Danish, Alshammari Abdullah S, Faiyaz Syed Shah Mohammed, Lila Amr Selim Abu, Katamesh Ahmed A, Khafagy El-Sayed, Alotaibi Hadil Faris, Ahmed Abo Bakr F
Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail P.O. Box 2440, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, University of Hail, Hail P.O. Box 2440, Saudi Arabia.
Nanomaterials (Basel). 2022 Oct 18;12(20):3643. doi: 10.3390/nano12203643.
Gold nanoparticles have gained popularity as an effective drug delivery vehicle due to their unique features. In fact, antibiotics transported via gold nanoparticles have significantly enhanced their potency in the recent past. The present study used an approach to synthesize gold nanoparticles in one step with the help of cefoxitin antibiotic as a reducing and stabilizing agent. Cefoxitin is a second-generation cephalosporin that loses its potential due to modification in the porins ( and ) of Gram-negative pathogens. Thus, the present study has developed an idea to revive the potential of cefoxitin against clinical Gram-negative pathogens, i.e., and , via applying gold nanoparticles as a delivery tool. Prior to antibacterial activity, characterization of cefoxitin-gold nanoparticles was performed via UV-visible spectrophotometry, dynamic light scattering, and electron microscopy. A characteristic UV-visible scan peak for gold nanoparticles was observed at 518 nm, ζ potential was estimated as -23.6 ± 1.6, and TEM estimated the size in the range of 2-12 nm. Moreover, cefoxitin loading efficiency on gold nanoparticles was calculated to be 71.92%. The antibacterial assay revealed that cefoxitin, after loading onto the gold nanoparticles, become potent against cefoxitin-resistant and , and their MIC values were estimated as 1.5 μg/mL and 2.5 μg/mL, respectively. Here, gold nanoparticles effectively deliver cefoxitin to the resistant pathogens, and convert it from unresponsive to a potent antibiotic. However, to obtain some convincing conclusions on the human relevance, their fate and toxicity need to be evaluated.
由于其独特的特性，金纳米颗粒作为一种有效的药物递送载体已受到广泛关注。事实上，近年来通过金纳米颗粒运输的抗生素显著增强了其效力。本研究采用一种方法，借助头孢西丁抗生素作为还原剂和稳定剂一步合成金纳米颗粒。头孢西丁是第二代头孢菌素，由于革兰氏阴性病原体孔蛋白（和）的修饰而失去其潜力。因此，本研究提出了一种想法，即通过应用金纳米颗粒作为递送工具来恢复头孢西丁对临床革兰氏阴性病原体（即和）的潜力。在进行抗菌活性测试之前，通过紫外可见分光光度法、动态光散射和电子显微镜对头孢西丁-金纳米颗粒进行了表征。在518nm处观察到金纳米颗粒的特征紫外可见扫描峰，ζ电位估计为-23.6±1.6，透射电子显微镜估计尺寸在2-12nm范围内。此外，计算得出头孢西丁在金纳米颗粒上的负载效率为71.92%。抗菌试验表明，头孢西丁负载到金纳米颗粒上后，对耐头孢西丁的和变得有效，其最低抑菌浓度值分别估计为1.5μg/mL和2.5μg/mL。在这里，金纳米颗粒有效地将头孢西丁递送至耐药病原体，并将其从不敏感转变为强效抗生素。然而，为了得出关于人体相关性的一些有说服力的结论，需要评估它们的命运和毒性。
