基于美国成年人控制喂养研究中的氨基酸碳同位素比值评估添加糖摄入量模型。

Evaluating a Model of Added Sugar Intake Based on Amino Acid Carbon Isotope Ratios in a Controlled Feeding Study of U.S. Adults.

机构信息

Institute of Arctic Biology, Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA.

College of Health Solutions, Arizona State University, Phoenix, AZ 85004, USA.

出版信息

Nutrients. 2022 Oct 14;14(20):4308. doi: 10.3390/nu14204308.

DOI:10.3390/nu14204308

PMID:36296992

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9611411/

Abstract

Previous studies suggest that amino acid carbon stable isotope ratios (CIRs) may serve as biomarkers of added sugar (AS) intake, but this has not been tested in a demographically diverse population. We conducted a 15-day feeding study of U.S. adults, recruited across sex, age, and BMI groups. Participants consumed personalized diets that resembled habitual intake, assessed using two consecutive 7-day food records. We measured serum ( = 99) CIRs collected at the end of the feeding period and determined correlations with diet. We used forward selection to model AS intake using participant characteristics and 15 CIRs. This model was internally validated using bootstrap optimism correction. Median (25th, 75th percentile) AS intake was 65.2 g/day (44.7, 81.4) and 9.5% (7.2%, 12.4%) of energy. The CIR of alanine had the highest, although modest, correlation with AS intake ( = 0.32, = 0.001). Serum CIRs were more highly correlated with animal food intakes, especially the ratio of animal to total protein. The AS model included sex, body weight and 6 CIRs. This model had modest explanatory power (multiple = 0.38), and the optimism-corrected was lower ( = 0.15). Further investigations in populations with wider ranges of AS intake are warranted.

摘要

先前的研究表明，氨基酸碳稳定同位素比值（CIRs）可能作为添加糖（AS）摄入量的生物标志物，但这尚未在人口统计学上多样化的人群中得到验证。我们对美国成年人进行了为期 15 天的喂养研究，招募对象涵盖了不同性别、年龄和 BMI 组。参与者食用了类似于习惯性摄入量的个性化饮食，这是通过连续两天的 7 天食物记录来评估的。我们测量了喂养期结束时采集的血清（=99）CIRs，并确定了与饮食的相关性。我们使用逐步选择法，使用参与者特征和 15 个 CIRs 来建立 AS 摄入量模型。该模型使用自举优化校正进行内部验证。AS 摄入量的中位数（25 分位，75 分位）为 65.2 克/天（44.7，81.4）和 9.5%（7.2%，12.4%）的能量。丙氨酸的 CIR 与 AS 摄入量的相关性最高（=0.32，=0.001），尽管相关性适中。血清 CIRs 与动物食物摄入量的相关性更高，尤其是动物蛋白与总蛋白的比值。AS 模型包括性别、体重和 6 个 CIRs。该模型具有适度的解释能力（多元=0.38），且经优化校正后的=0.15。在 AS 摄入量范围更广的人群中进一步研究是必要的。