利用多间隔序列分型法对伊朗[具体研究对象]的遗传多样性研究 。（这里原文不完整，“of”后面应该还有具体所研究的事物）
Genetic Diversity of in Iran by Multi-Spacer Sequence Typing.
作者信息
Mohabati Mobarez Ashraf, Baseri Neda, Khalili Mohammad, Mostafavi Ehsan, Stenos John, Esmaeili Saber
机构信息
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 1411713116, Iran.
Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran 1316943551, Iran.
出版信息
Pathogens. 2022 Oct 11;11(10):1175. doi: 10.3390/pathogens11101175.
, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST genotypes among Iranian domestic ruminants. Understanding the genotypic profiles is critical in determining and preventing Q fever outbreaks.
Q热的人畜共患病原体在包括伊朗在内的全球范围内均有分布。然而，伊朗尚未有关于这种感染的流行基因型的相关报道。本研究旨在使用多间隔序列分型（MST）方法评估伊朗Q热病原体的遗传多样性。首先，使用针对该病原体基因的定量聚合酶链反应（qPCR）对14份阳性样本（从4只绵羊、3只山羊和7头牛采集）进行了确认。然后，使用PCR扩增了10个间隔序列（Cox 2、5、18、20、22、37、51、56、57和61），以备将来进行MST分析。对家养反刍动物样本的MST基因分型分析揭示了Cox5和Cox56位点的两个新等位基因（Cox5.11和Cox56.15），这导致了四种新的MST基因型（MST62、63、64和65）的出现以及一种先前已描述的MST基因型（MST61）。本研究表明伊朗家养反刍动物中存在五种MST基因型。了解该病原体的基因型谱对于确定和预防Q热疫情至关重要。
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