对西班牙北部家养反刍动物伯氏考克斯体的基因分型。
Genotyping of Coxiella burnetii from domestic ruminants in northern Spain.
机构信息
NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Department of Animal Health, Derio, Bizkaia, Spain.
出版信息
BMC Vet Res. 2012 Dec 10;8:241. doi: 10.1186/1746-6148-8-241.
BACKGROUND
Information on the genotypic diversity of Coxiella burnetii isolates from infected domestic ruminants in Spain is limited. The aim of this study was to identify the C. burnetii genotypes infecting livestock in Northern Spain and compare them to other European genotypes. A commercial real-time PCR targeting the IS1111a insertion element was used to detect the presence of C. burnetii DNA in domestic ruminants from Spain. Genotypes were determined by a 6-loci Multiple Locus Variable number tandem repeat analysis (MLVA) panel and Multispacer Sequence Typing (MST).
RESULTS
A total of 45 samples from 4 goat herds (placentas, N = 4), 12 dairy cattle herds (vaginal mucus, individual milk, bulk tank milk, aerosols, N = 20) and 5 sheep flocks (placenta, vaginal swabs, faeces, air samples, dust, N = 21) were included in the study. Samples from goats and sheep were obtained from herds which had suffered abortions suspected to be caused by C. burnetii, whereas cattle samples were obtained from animals with reproductive problems compatible with C. burnetii infection, or consisted of bulk tank milk (BTM) samples from a Q fever surveillance programme. C. burnetii genotypes identified in ruminants from Spain were compared to those detected in other countries. Three MLVA genotypes were found in 4 goat farms, 7 MLVA genotypes were identified in 12 cattle herds and 4 MLVA genotypes were identified in 5 sheep flocks. Clustering of the MLVA genotypes using the minimum spanning tree method showed a high degree of genetic similarity between most MLVA genotypes. Overall 11 different MLVA genotypes were obtained corresponding to 4 different MST genotypes: MST genotype 13, identified in goat, sheep and cattle from Spain; MST genotype 18, only identified in goats; and, MST genotypes 8 and 20, identified in small ruminants and cattle, respectively. All these genotypes had been previously identified in animal and human clinical samples from several European countries, but some of the MLVA genotypes are described here for the first time.
CONCLUSIONS
Genotyping revealed a substantial genetic diversity among domestic ruminants from Northern Spain.
背景
关于西班牙感染家畜的柯克斯体分离株的基因型多样性信息有限。本研究的目的是确定感染西班牙北方家畜的伯氏考克斯体基因型，并将其与其他欧洲基因型进行比较。使用针对 IS1111a 插入元件的商业实时 PCR 检测来自西班牙的家畜中是否存在伯氏考克斯体 DNA。通过 6 个基因座多位点可变数串联重复分析（MLVA）面板和多间隔序列分型（MST）确定基因型。
结果
本研究共纳入 45 个样本，分别来自 4 个山羊群（胎盘，N=4）、12 个奶牛群（阴道黏液、个体奶、奶桶奶、气溶胶、N=20）和 5 个绵羊群（胎盘、阴道拭子、粪便、空气样本、灰尘、N=21）。来自山羊和绵羊的样本来自怀疑因伯氏考克斯体而流产的羊群，而牛的样本来自与伯氏考克斯体感染相符的生殖问题动物，或来自 Q 热监测计划的奶桶奶样本。从西班牙家畜中鉴定的伯氏考克斯体基因型与在其他国家检测到的基因型进行了比较。在 4 个山羊场发现了 3 种 MLVA 基因型，在 12 个奶牛群中发现了 7 种 MLVA 基因型，在 5 个绵羊群中发现了 4 种 MLVA 基因型。使用最小生成树法对 MLVA 基因型进行聚类，显示大多数 MLVA 基因型之间具有高度的遗传相似性。总体上获得了 11 种不同的 MLVA 基因型，对应 4 种不同的 MST 基因型：在西班牙的山羊、绵羊和牛中发现的 MST 基因型 13；仅在山羊中发现的 MST 基因型 18；以及分别在小反刍动物和牛中发现的 MST 基因型 8 和 20。所有这些基因型以前都在来自几个欧洲国家的动物和人类临床样本中被鉴定出来，但有些 MLVA 基因型是首次在西班牙家畜中被鉴定出来。
结论
基因分型显示，来自西班牙北方的家畜具有显著的遗传多样性。
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