Khedr Amgad I M, Farrag Abdelaziz F S, Nasr Ali M, Swidan Shady A, Nafie Mohamed S, Abdel-Kader Maged S, Goda Marwa S, Badr Jihan M, Abdelhameed Reda F A
Department of Pharmacognosy, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt.
Department of Pharmaceutics, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt.
Pharmaceutics. 2022 Oct 13;14(10):2185. doi: 10.3390/pharmaceutics14102185.
Different parts of L. and their green synthesized eco-friendly silver nanoparticles (AgNPs) were screened for their cytotoxicity and apoptotic activity. Results showed that flower extract AgNPs exhibited more potent cytotoxicity compared to the normal form against PC-3 and A549 cell lines with IC values of 2.47 μg/mL and 1.35 μg/mL, respectively. The results were compared to doxorubicin (IC = 5.13 and 6.19 μg/mL, respectively). For apoptosis-induction, AgNPs prepared from the flower extract induced cell death by apoptosis by 41.34-fold change and induced necrotic cell death by 10.2-fold. Additionally, they induced total prostate apoptotic cell death by a 16.18-fold change, and it slightly induced necrotic cell death by 2.7-fold. Hence, green synthesized flower extract AgNPs exhibited cytotoxicity in A549 and PC-3 through apoptosis-induction in both cells. Consequently, synthesized AgNPs were further tested for apoptosis and increased gene and protein expression of pro-apoptotic markers while decreasing expression of anti-apoptotic genes. As a result, this formula may serve as a promising source for anti-cancer candidates. Finally, liquid chromatography combined with electrospray mass spectrometry (LC-ESI-MS) analysis was assessed to identify the common bioactive metabolites in crude extracts of stem, flower, and bract.
对L.的不同部位及其绿色合成的环保型银纳米颗粒（AgNPs）进行了细胞毒性和凋亡活性筛选。结果表明，与正常形态相比，花提取物AgNPs对PC-3和A549细胞系表现出更强的细胞毒性，IC值分别为2.47μg/mL和1.35μg/mL。将结果与阿霉素（IC分别为5.13和6.19μg/mL）进行比较。对于凋亡诱导，由花提取物制备的AgNPs通过凋亡诱导细胞死亡的变化倍数为41.34倍，诱导坏死性细胞死亡的变化倍数为10.2倍。此外，它们通过16.18倍的变化诱导前列腺细胞总凋亡死亡，并轻微诱导坏死性细胞死亡，变化倍数为2.7倍。因此，绿色合成的花提取物AgNPs通过诱导两种细胞的凋亡在A549和PC-3中表现出细胞毒性。因此，对合成的AgNPs进行了进一步的凋亡测试，并检测了促凋亡标志物的基因和蛋白表达增加，同时抗凋亡基因的表达降低。结果，该配方可能成为抗癌候选物的有希望来源。最后，评估了液相色谱结合电喷雾质谱（LC-ESI-MS）分析，以鉴定茎、花和苞片粗提物中的常见生物活性代谢物。
