Zhao Pan, Wang Jigang, Zou Chang
School of Medicine, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, China.
Department of Central Laboratory, Shenzhen People's Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Front Pharmacol. 2025 Apr 3;16:1565308. doi: 10.3389/fphar.2025.1565308. eCollection 2025.
has demonstrated promising therapeutic potential due to its bioactive compounds. This study investigated the cytotoxic and pro-apoptotic effects of extract, its active fraction, and biosynthesized silver nanoparticles (AgNPs) on human gastric cancer (HGT-1) cells.
The plant was collected and subjected to Soxhlet extraction using ethanol, followed by sequential solvent partitioning and silica gel column chromatography to isolate bioactive fractions. Green synthesis of AgNPs was conducted using extract. Structural characterization was conducted via UV- spectroscopy, FTIR, XRD, and FESEM. Cytotoxicity was assessed using MTT and CCK-8 assays, while apoptosis induction was evaluated through qPCR and Western blot analysis of key apoptotic markers.
The ethanolic extract exhibited moderate cytotoxicity against HGT-1 cells, whereas biosynthesized AgNPs demonstrated enhanced anticancer activity with reduced toxicity to normal hepatocytes. The active fraction, identified as cryptomeridiol, showed the highest selectivity and potency against cancer cells. qPCR revealed significant upregulation of p21 and downregulation of CDK2, suggesting cell cycle arrest. Western blot analysis confirmed increased expression of caspase-3 and caspase-9 and a reduction in XIAP, indicating apoptosis activation.
This study underscores the potential of bioactive compounds and AgNPs as therapeutic agents, particularly against gastric cancer. The findings provide a basis for further exploration into their mechanism of action and broader pharmacological applications. Keywords: , Bioactive compounds, Silver nanoparticles (AgNPs), Gastric cancer-HGT-1, Apoptosis.
由于其生物活性化合物，已显示出有前景的治疗潜力。本研究调查了[植物名称]提取物、其活性成分以及生物合成的银纳米颗粒（AgNPs）对人胃癌（HGT - 1）细胞的细胞毒性和促凋亡作用。
采集该植物，用乙醇进行索氏提取，随后进行连续溶剂分配和硅胶柱色谱以分离生物活性成分。使用[植物名称]提取物进行AgNPs的绿色合成。通过紫外光谱、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和场发射扫描电子显微镜（FESEM）进行结构表征。使用MTT和CCK - 8测定法评估细胞毒性，同时通过关键凋亡标志物的定量聚合酶链反应（qPCR）和蛋白质免疫印迹分析评估凋亡诱导情况。
乙醇提取物对HGT - 1细胞表现出中等细胞毒性，而生物合成的AgNPs显示出增强的抗癌活性且对正常肝细胞毒性降低。鉴定为柳杉二醇的活性成分对癌细胞表现出最高的选择性和效力。qPCR显示p21显著上调，CDK2下调，表明细胞周期停滞。蛋白质免疫印迹分析证实caspase - 3和caspase - 9表达增加，X连锁凋亡抑制蛋白（XIAP）减少，表明凋亡激活。
本研究强调了[植物名称]生物活性化合物和AgNPs作为治疗剂的潜力，特别是对胃癌。这些发现为进一步探索其作用机制和更广泛的药理学应用提供了基础。关键词：[植物名称]、生物活性化合物、银纳米颗粒（AgNPs）、胃癌 - HGT - 1、凋亡。
