Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The British University in Egypt, El-Sherouk City, Egypt.
J Biomol Struct Dyn. 2024;42(22):12077-12089. doi: 10.1080/07391102.2023.2267666. Epub 2023 Oct 10.
Crude or semi-purified extracts of plants can play a significant role as antitumor agents. They were used as stabilizing and reducing agents in the preparation of silver nanoparticles (AgNPs) that allows these particles to have more efficient cytotoxic activity. In the current study, the extract of L., a plant of common occurrence in Egypt was used to synthesize AgNPs for the first time, where comparison of anticancer activity of crude and phenolic extracts with the AgNPs were extensively studied against cancer cell lines PC-3 and HCT-116. Interestingly, AgNPs of the crude extract exhibited promising cytotoxicity with IC values of 10.4 and 16.3 μg/ml, while AgNPs of the phenolic extract exhibited very potent cytotoxicity with IC values of 2.66 and 1.34 μg/ml compared to Doxorubicin (as a standard reference drug) that exhibited IC values of 5.13 and 4.36 μg/ml, respectively against the tested cells. Additionally, AgNPs of the phenolic extract induced apoptosis in HCT-116 with a higher ratio than in PC-3 cells. It induced apoptosis in PC-3 cells by 79.3-fold change, while it induced total colon apoptotic cell death by 228.3-fold change compared to untreated control. Finally, the apoptotic activity of AgNPs of the phenolic extract in the treated PC-3 and HCT-116 cells was confirmed using RT-PCR. As a result, AgNPs of the phenolic extract could be considered a promising anticancer candidate through apoptosis-induction.Communicated by Ramaswamy H. Sarma.
植物的粗提物或半纯化提取物可以作为抗肿瘤剂发挥重要作用。它们被用作银纳米粒子（AgNPs）制备中的稳定剂和还原剂，这使得这些粒子具有更有效的细胞毒性活性。在当前的研究中，首次使用埃及常见的 L.植物提取物来合成 AgNPs，其中对粗提物和酚类提取物与 AgNPs 的抗癌活性进行了广泛比较，研究了它们对 PC-3 和 HCT-116 癌细胞系的影响。有趣的是，粗提物 AgNPs 表现出有希望的细胞毒性，IC 值为 10.4 和 16.3μg/ml，而酚类提取物 AgNPs 表现出非常强的细胞毒性，IC 值分别为 2.66 和 1.34μg/ml，与多柔比星（作为标准参考药物）相比，对测试细胞的 IC 值分别为 5.13 和 4.36μg/ml。此外，酚类提取物 AgNPs 在 HCT-116 中诱导的细胞凋亡比例高于 PC-3 细胞。它在 PC-3 细胞中诱导了 79.3 倍的凋亡变化，而与未处理的对照组相比，它在总结肠凋亡细胞死亡中诱导了 228.3 倍的变化。最后，使用 RT-PCR 证实了酚类提取物 AgNPs 在处理的 PC-3 和 HCT-116 细胞中的凋亡活性。因此，酚类提取物 AgNPs 可以通过诱导细胞凋亡被认为是一种很有前途的抗癌候选物。由 Ramaswamy H. Sarma 传达。
