Alhadrami Hani A, Sayed Ahmed M, Hassan Hossam M, Rateb Mostafa E, Abdelkader Karim
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80402, Jeddah 21589, Saudi Arabia.
Molecular Diagnostic Laboratory, King Abdulaziz University Hospital, King Abdulaziz University, P.O. Box 80402, Jeddah 21589, Saudi Arabia.
Pharmaceutics. 2022 Oct 16;14(10):2201. doi: 10.3390/pharmaceutics14102201.
The prevalence of multidrug-resistant among animal- and plant-derived food products threatens global healthcare and economic sectors. Hen egg white lysozyme is widely exploited as a food preservative against Gram-positive pathogens. Nevertheless, its limited penetration of the outer membrane renders it ineffective against Gram-negative bacteria. Herein, we present a safe and effective approach to facilitate HEWL access to peptidoglycan layers using cecropin A. In silico analysis of cecropin A peptide revealed an amphipathic α-helical peptide with potential outer membrane permeabilizing activity through its interaction with both hydrophobic and ionic stabilizing forces. Evaluation of HEWL/cecropin A combination showed a cecropin A dose-dependent bacterial count reduction up to 4.16 and 3.18 ± 0.26 log units against ATCC 35664 at the logarithmic and stationary growth phases, respectively. Moreover, the combination displayed antibacterial activity of 2.1 ± 0.31 and ~1 log-unit reductions against serovars Kentucky, Typhimurium, and Enteritidis, respectively, whereas Hato and Shangani were found irresponsive. The cytotoxicity assay revealed compatibility of cecropin A with oral epithelial cells. These observations suggest HEWL/cecropin A combination as an effective and safe alternative to lysozyme against .
动植物源性食品中多重耐药性的流行威胁着全球医疗保健和经济部门。鸡蛋清溶菌酶被广泛用作抗革兰氏阳性病原体的食品防腐剂。然而，其对外膜的穿透有限，使其对革兰氏阴性菌无效。在此，我们提出了一种安全有效的方法，利用天蚕素A促进溶菌酶进入肽聚糖层。对天蚕素A肽的计算机模拟分析揭示了一种两亲性α-螺旋肽，通过其与疏水和离子稳定力的相互作用具有潜在的外膜通透活性。对溶菌酶/天蚕素A组合的评估表明，在对数生长期和稳定生长期，天蚕素A剂量依赖性地使针对ATCC 35664的细菌计数分别减少高达4.16和3.18±0.26个对数单位。此外，该组合对肯塔基、鼠伤寒和肠炎血清型分别显示出2.1±0.31和~1个对数单位的抗菌活性降低，而哈托和尚加尼血清型则无反应。细胞毒性试验表明天蚕素A与口腔上皮细胞具有相容性。这些观察结果表明，溶菌酶/天蚕素A组合是一种针对……的有效且安全的溶菌酶替代物。
