Zhou Meiqi, Peng Nafei, Yang Chuanping, Wang Chao
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Plants (Basel). 2022 Oct 13;11(20):2700. doi: 10.3390/plants11202700.
Dehydrin (DHN), a member of the late embryogenesis abundant protein (LEA) family, was recently found to play a role in physiological responses to salt and drought stress. In this study, we identified and cloned the gene from . The PtrDHN-3 protein encoded 226 amino acids, having a molecular weight of 25.78 KDa and an isoelectric point of 5.18. It was identified as a SKn-type DHN and was clustered with other resistance-related DHN proteins. Real-time fluorescent quantitative PCR showed that transcription levels of were induced by mannitol stress, and more significantly by salt stress. Meanwhile, in a yeast transgenic assay, salt tolerance increased in the transgenic yeast, while the germination rate, fresh weight and chlorophyll content increased in -overexpressing transgenic plants (OE) under salt stress. Significant increases in expression levels of six antioxidant enzymes genes, and SOD and POD enzyme activity was also observed in the OE lines, resulting in a decrease in O and HO accumulation. The proline content also increased significantly compared with the wild-type, along with expression of proline synthesis-related genes and . These findings suggest that plays an important role in salt resistance in plants.
脱水素（DHN）是胚胎发育晚期丰富蛋白（LEA）家族的一员，最近发现其在植物对盐和干旱胁迫的生理响应中发挥作用。在本研究中，我们从[具体来源未给出]中鉴定并克隆了该基因。PtrDHN - 3蛋白由226个氨基酸编码，分子量为25.78 kDa，等电点为5.18。它被鉴定为SKn型脱水素，并与其他抗性相关的脱水素蛋白聚类。实时荧光定量PCR表明，[基因名称未给出]的转录水平受甘露醇胁迫诱导，受盐胁迫诱导更为显著。同时，在酵母转基因试验中，转[基因名称未给出]酵母的耐盐性增强，而在盐胁迫下，过表达[基因名称未给出]的转基因[植物名称未给出]植株（OE）的发芽率、鲜重和叶绿素含量增加。在OE株系中还观察到六种抗氧化酶基因的表达水平显著增加，以及超氧化物歧化酶（SOD）和过氧化物酶（POD）的酶活性增加，导致超氧阴离子（O₂⁻）和过氧化氢（H₂O₂）积累减少。与野生型相比，脯氨酸含量也显著增加，同时脯氨酸合成相关基因[基因名称未给出]和[基因名称未给出]的表达也增加。这些结果表明[基因名称未给出]在植物抗盐性中起重要作用。
